Plant-microbe association for rhizoremediation of chloronitroaromatic pollutants with Comamonas sp strain CNB-1
Plant-microbe association for rhizoremediation of chloronitroaromatic pollutants with Comamonas sp strain CNB-1
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DOI：
10.1111/j.1462-2920.2006.01163.x
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发表时间：
2007-02-01
影响因子：
5.1
通讯作者：
Liu, Shuang-Jiang
中科院分区：
文献类型：
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作者：
Liu, Lei;Jiang, Cheng-Ying;Liu, Shuang-Jiang
从活性污泥中分离出一株对4-氯硝基苯（4CNB）具有较强降解能力的Comamonas spp . CNB-1菌株，通过与紫花苜蓿的结合对4CNB污染的土壤进行根根修复。共聚焦激光扫描显微镜显示菌株CNB-1成功定植苜蓿根。采用培养法和针对氯硝基苯硝基还原酶基因的定量竞争PCR技术测定菌株CNB-1的种群数量，结果表明，菌株CNB-1在根际的种群数量比在散装土壤中的种群数量高出10-100倍。实验结果表明，在土壤中施用4CNB后1 ~ 2 d内，污染物4CNB被完全去除，接种菌株CNB-1可消除其对苜蓿的植物毒性。pcr -变性梯度凝胶电泳和16S rRNA基因库分析结果显示，土壤微生物群落主要由α -变形菌门、β -变形菌门、γ -变形菌门、CFB菌（cytophaga - flavabacteria - bacteriodes）和酸性菌门组成。接种菌株CNB-1对该微生物群落影响不显著。因此，本研究建立了一套对4CNB进行根治的共花草-紫花苜蓿体系。
Comamonas sp. strain CNB-1, isolated from activated sludge and having a strong ability to degrade 4-chloronitrobenzene (4CNB), was applied for rhizoremediation of 4CNB-polluted soil through association with alfalfa. Confocal laser scanning microscopy revealed that strain CNB-1 successfully colonized alfalfa roots. Determination of strain CNB-1 populations by cultivation method and by quantitative competitive PCR technique targeting the chloronitrobenzene nitroreductase gene showed that the population of strain CNB-1 in the rhizosphere was about 10-100 times higher than that in the bulk soil. Gnotobiotic and outdoor experiments showed that pollutant 4CNB was completely removed within 1 or 2 days after 4CNB application into soil, and that its phytotoxicity to alfalfa was eliminated by inoculation of strain CNB-1. Results from PCR-denaturing gradient gel electrophoresis and analysis of 16S rRNA gene libraries revealed that the indigenous soil microbial community mainly consisted of alphaproteobacteria, betaproteobacteria, gammaproteobacteria, the CFB bacteria (Cytophaga-Flavabacterium-Bacteriodes), and Acidobacteria. This microbial community was not significantly influenced by inoculation of strain CNB-1. Thus, this study has developed a Comamonas-alfalfa system for rhizoremediation of 4CNB.