The phylogenetic composition and structure of soil microbial communities shifts in response to elevated carbon dioxide
The phylogenetic composition and structure of soil microbial communities shifts in response to elevated carbon dioxide
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DOI：
10.1038/ismej.2011.99
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发表时间：
2012-02-01
期刊：
影响因子：
11
通讯作者：
Zhou, Jizhong
中科院分区：
文献类型：
--
作者：
He, Zhili;Piceno, Yvette;Zhou, Jizhong
与全球变化有关的主要因素之一是大气中二氧化碳浓度不断增加。虽然已经确定了CO2浓度升高对植物生长和初级生产力的刺激作用，但其对土壤微生物群落多样性和功能的影响却知之甚少。在这项研究中，系统发育芯片（PhyloChip）被用来全面调查在两种CO2条件下（环境，368 p. p.m.，相对于升高的560 p. p.m.）10年在eCO下，基于检测到的操作分类单位（OTU）数量的丰富度显著下降（2）。PhyloChip共检测到2269个OTU，分别来自45门（其中2个来自海洋）、55纲、99目、164科和190亚科。此外，五个门的信号强度（Crenarchaeota，Chloroflexi，OP 10，OP 9/JS 1，Verrucomicrobia）在eCO（2）下显著降低，并且eCO（2）对微生物组成的这种显著影响也在最丰富的门的纲或更低分类水平上观察到，如变形菌门，厚壁菌门，放线菌门，拟杆菌门和酸杆菌门，这表明在eCO（2）下微生物群落组成发生了变化。此外，统计分析表明，在eCO（2）下，土壤微生物群落的整体分类结构发生了变化。Mantel试验表明，土壤微生物群落的物种丰富度、组成和结构的变化与土壤和植物性质密切相关。这项研究为我们理解eCO（2）下土壤微生物群落的丰富度，组成和结构的变化以及塑造微生物群落结构的环境因素提供了见解。The ISME Journal（2012）6，259-272; doi：10.1038/ismej.2011.99; 2011年7月28日在线发表
One of the major factors associated with global change is the ever-increasing concentration of atmospheric CO2. Although the stimulating effects of elevated CO2 (eCO(2)) on plant growth and primary productivity have been established, its impacts on the diversity and function of soil microbial communities are poorly understood. In this study, phylogenetic microarrays (PhyloChip) were used to comprehensively survey the richness, composition and structure of soil microbial communities in a grassland experiment subjected to two CO2 conditions (ambient, 368 p. p. m., versus elevated, 560 p. p. m.) for 10 years. The richness based on the detected number of operational taxonomic units (OTUs) significantly decreased under eCO(2). PhyloChip detected 2269 OTUs derived from 45 phyla (including two from Archaea), 55 classes, 99 orders, 164 families and 190 subfamilies. Also, the signal intensity of five phyla (Crenarchaeota, Chloroflexi, OP10, OP9/JS1, Verrucomicrobia) significantly decreased at eCO(2), and such significant effects of eCO(2) on microbial composition were also observed at the class or lower taxonomic levels for most abundant phyla, such as Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes and Acidobacteria, suggesting a shift in microbial community composition at eCO(2). Additionally, statistical analyses showed that the overall taxonomic structure of soil microbial communities was altered at eCO(2). Mantel tests indicated that such changes in species richness, composition and structure of soil microbial communities were closely correlated with soil and plant properties. This study provides insights into our understanding of shifts in the richness, composition and structure of soil microbial communities under eCO(2) and environmental factors shaping the microbial community structure. The ISME Journal (2012) 6, 259-272; doi: 10.1038/ismej.2011.99; published online 28 July 2011