Alternative polyadenylation events differ dramatically between Tongcheng and Large White pigs in response to PRRSV infection.
Alternative polyadenylation events differ dramatically between Tongcheng and Large White pigs in response to PRRSV infection.
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桐城猪和大白猪响应 PRRSV 感染的替代多聚腺苷酸化事件存在显着差异。
DOI：
10.1111/age.13125
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发表时间：
2021-07
期刊：
影响因子：
2.4
通讯作者：
Zhou X
中科院分区：
文献类型：
--
作者：
Meng X;Kuang K;Zhang Y;Guan K;Liu B;Zhou X
选择性多聚腺苷酸化(APA)是一种广泛存在的转录后调控机制，增加了转录组和蛋白质组的生物学复杂性。然而，目前尚不清楚APA调控是否在猪繁殖与呼吸综合征病毒(PRRSV)的遗传抗性中起作用。在此，我们报道了抗PRRSV通城(TC)猪和敏感PRRSV大白猪(LW)感染PRRSV后全基因组APA对猪肺泡巨噬细胞的调节。采用3‘m RNA测序策略，在TC和LW猪肺泡巨噬细胞中检测到75-981个高质量的APA位点。此外，在感染PRRSV的TC猪和LW猪中，分别发现了1202和1089个差异表达的APA位点，以及79和117个非翻译区APA转换基因。TC猪和LW猪的APA事件涉及不同的生物学途径，而TC猪的APA事件与PRRSV感染的免疫应答直接相关。此外，我们还鉴定了影响TC猪和LW猪多聚腺苷信号的遗传变异。这些发现将为进一步了解PRRSV的遗传抗性提供有用的APA调控信息。
Alternative polyadenylation (APA) is a widespread post-transcriptional regulation mechanism that increases the biological complexity of transcriptome and proteome. However, it is unclear whether APA regulation plays a role in genetic resistance to porcine reproductive and respiratory syndrome virus (PRRSV). Here, we reported genome-wide APA regulation of porcine alveolar macrophages in PRRSV-resistant Tongcheng (TC) pigs and PRRSV-susceptible Large White (LW) pigs upon PRRSV infection. Using 3' mRNA sequencing strategy, we detected 75 981 high-quality APA sites in porcine alveolar macrophages of TC and LW pigs. Furthermore, 1202 and 1089 differentially expressed APA sites, as well as 79 and 117 untranslated region-APA switching genes were identified in TC pigs and LW pigs upon PRRSV infection respectively. The APA events in TC pigs and LW pigs were involved in different biological pathways, while APA events in TC pigs are directly associated with the immune response to PRRSV infection. In addition, we identified genetic variations affecting polyadenylation signal between TC pigs and LW pigs. These findings would provide helpful information on APA regulation for further understanding of genetic resistance to PRRSV.
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DOI：
10.1016/b978-0-12-809633-8.20106-4
发表时间：
2020-02
期刊：
--
影响因子：
--
作者：
A. Prjibelski;Anton I. Korobeynikov;Alla L. Lapidus
通讯作者：
A. Prjibelski;Anton I. Korobeynikov;Alla L. Lapidus
热带爪蟾全基因组多腺苷酸化信号的建模
影响因子：
3.7
作者：
Zhu Sheng;Wu Xiaohui;Fu Hongjuan;Ye Congting;Chen Moliang;Jiang Zhihua;Ji Guoli
通讯作者：
Ji Guoli
影响因子：
7
作者：
Ulitsky I;Shkumatava A;Jan CH;Subtelny AO;Koppstein D;Bell GW;Sive H;Bartel DP
通讯作者：
Bartel DP
影响因子：
14.9
作者：
Tian B;Hu J;Zhang H;Lutz CS
通讯作者：
Lutz CS
影响因子：
30.8
作者：
Li, Lei;Huang, Kai-Lieh;Li, Wei
通讯作者：
Li, Wei