Comparative morphological study of zygotic and microspore-derived embryos of Brassica napus L. as revealed by scanning electron microscopy
Comparative morphological study of zygotic and microspore-derived embryos of Brassica napus L. as revealed by scanning electron microscopy
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DOI：
10.1006/anbo.1998.0661
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发表时间：
1998-08-01
期刊：
影响因子：
4.2
通讯作者：
Fowke, LC
中科院分区：
文献类型：
--
作者：
Ilic-Grubor, K;Attree, SM;Fowke, LC
甘蓝型油菜小孢子胚和合子胚的比较形态学研究进行，说明这些胚胎在整个发育过程中的外部形态基本相似。用高分子量聚乙二醇（PEG）代替蔗糖作为培养基，从分离的小孢子中产生单倍体胚。形态变化的时间过程中诱导的PEG（25%）和蔗糖（13%）的小孢子胚胎发育的详细描述，通过扫描电子显微镜（SEM）显示，并比较相应阶段的合子胚在排卵。在心脏，鱼雷和早期子叶阶段，小孢子衍生（MD）胚胎PEG密切类似于他们的合子。相反，在高蔗糖培养基上诱导的胚的外部形态不同于PEG和合子胚的外部形态，表明培养中高浓度的蔗糖对B中MD胚的发育具有形态发生效应。油菜。在MD胚的整个发育过程中，在根端和胚柄顶端经常观察到原始花粉壁的碎片。这表明，极性在MD胚可能起源于结构极化后期单核小孢子和早期的双细胞花粉。(C)1998年《植物学年鉴》（Annals of Botany Company）
A comparative morphological study of microspore-derived (MD) and zygotic embryos of Brassica napus L. was conducted, illustrating substantial similarities in external morphology of these embryos throughout their development. Haploid embryos were produced from isolated microspores cultured on high molecular weight polyethylene glycol (PEG), replacing sucrose as an osmoticum. Morphological changes during the time-course of microspore embryo development induced on PEG (25%) and sucrose (13%) are described in detail as revealed by scanning electron microscopy (SEM) and compared to the corresponding stages of zygotic embryos developed in ovulo. At the heart, torpedo and early cotyledonary stages, microspore-derived (MD) embryos on PEG closely resemble their zygotic counterparts. In contrast, the external morphology of embryos induced on high sucrose medium differs from that of PEG and zygotic embryos indicating that a high concentration of sucrose in culture has a morphogenetic effect on MD embryo development in B. napus. Fragments of the original pollen wall are regularly observed at the root pole region and at the tips of suspensors in MD embryos throughout their development. This suggests that polarity in MD embryos might originate from structurally polarized late uninuclear microspores and early bicellular pollen. (C) 1998 Annals of Botany Company.