Fecal short-chain fatty acid concentrations and effect on ileal pouch function.
Fecal short-chain fatty acid concentrations and effect on ileal pouch function.
复制标题
粪便短链脂肪酸浓度及其对回肠袋功能的影响。
DOI：
10.1007/bf02053503
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发表时间：
1993
影响因子：
3.9
通讯作者：
Haddad,AC
中科院分区：
文献类型：
--
作者：
AmbrozeJr,WL;Pemberton,JH;Phillips,SF;Bell,AM;Haddad,AC
：手术后 43+/-5（平均值+/-SEM）个月从 14 名回肠储袋肛门吻合术 (IPAA) 患者中随机采集粪便样本，并通过气相色谱法测定各个短链脂肪酸 (SCFA) 的浓度。大便频率是根据粪便取样前 15 天记录的日记确定的。将生理浓度的 SCFA 和生理盐水随机注入 6 名 IPAA 患者的造袋后，通过测压法确定造袋内阶段性收缩 (PC) 的频率、幅度和持续时间。 IPAA 后的平均总 SCFA 浓度与正常粪便没有显着差异（IPAA 后为 83+/-20 mM，对照为 97+/-10 mM；P> 0.05）。在 IPAA 患者中，回归分析表明每日粪便与总 SCFA 浓度之间呈反比关系 (r= 0.73；P< 0.001)。此外，与生理盐水对照相比，SCFA输注后PC的频率（3.0+/-0.9 vs. 3.2+/-0.8 PCs/30 分钟）、幅度（26+/-5 vs. 25+/-4 mmHg）或持续时间（23+/-3 vs. 26+/-2 秒）没有变化（P>0.1）。这些发现表明，回肠贮袋排出物中存在 SCFA，并且粪便频率可能与粪便 SCFA 浓度有关。此外，回肠末端对短链脂肪酸 SCFA 的正常收缩反应不会发生在回肠囊中。
: Random stool samples were obtained from 14 ileal pouch-anal anastomosis (IPAA) patients 43+/-5 (mean+/-SEM) months after surgery, and the concentrations of individual short-chain fatty acids (SCFAs) were determined by gas liquid chromatography. Stool frequency was determined from a diary recorded for 15 days prior to stool sampling. The frequency, amplitude, and duration of phasic contractions (PCs) within the pouch following infusion of a physiologic concentration of SCFAs and normal saline randomly into the pouch of six IPAA patients were determined manometrically. The mean total SCFA concentration after IPAA did not differ significantly from normal stools (83+/-20 mM after IPAA vs. 97+/-10 mM for controls; P> 0.05). In the IPAA patients, regression analysis demonstrated an inverse relationship between stools per day and total SCFA concentration (r= 0.73; P< 0.001). Moreover, no change in frequency (3.0+/-0.9 vs. 3.2+/-0.8 PCs/30 minutes), amplitude (26+/-5 vs. 25+/-4 mmHg), or duration (23+/-3 vs. 26+/-2 seconds) of PCs was found after SCFA infusion compared with saline control (P> 0.1). These findings demonstrate that SCFAs are present in ileal pouch effluent and that stool frequency may be related to fecal SCFA concentration. Also, the normal contractile response of the terminal ileum to SCFAs does not occur in the ileal pouch.