Identical Hik-Rre systems are involved in perception and transduction of salt signals and hyperosmotic signals but regulate the expression of individual genes to different extents in Synechocystis
Identical Hik-Rre systems are involved in perception and transduction of salt signals and hyperosmotic signals but regulate the expression of individual genes to different extents in Synechocystis
复制标题
DOI：
10.1074/jbc.m412174200
复制
发表时间：
2005-06-03
影响因子：
4.8
通讯作者：
Murata, N
中科院分区：
文献类型：
--
作者：
Shumskaya, MA;Paithoonrangsarid, K;Murata, N
在以前的研究中，我们的特点是五个组氨酸激酶（Hiks）和同源反应调节（Rres），控制类似于70%的高渗胁迫诱导的基因在蓝藻集胞藻属PCC 6803的表达。在本研究中，我们通过RNA狭缝印迹杂交和全基因组DNA微阵列筛选了Rres基因敲除文库，并确定了三个Hik-Rre系统，即Hik 33-Rre 31，Hik 10-Rre 3和Hik 16-Hik 41-Rre 17，以及另一个系统，其中包括Rre 1，参与感知盐胁迫和信号转导。我们发现这些Hik-Rre系统与参与高渗应激信号感知和转导的系统相同。我们比较了盐胁迫和高渗胁迫诱导基因的诱导因子，发现这些基因对两种胁迫的响应程度不同。此外，Hik 33-Rre 31系统调节由高渗胁迫特异性诱导的基因的表达，而包括Rre 1的系统调节由盐胁迫或高渗胁迫特异性诱导的一个或两个基因的表达。我们的观察结果表明，盐和高渗胁迫的Hik-Rre系统的感知是复杂的，盐胁迫和高渗胁迫被视为不同的信号的Hik-Rre系统。
In previous studies, we characterized five histidine kinases (Hiks) and the cognate response regulators (Rres) that control the expression of similar to 70% of the hyperosmotic stress-inducible genes in the cyanobacterium Synechocystis sp. PCC 6803. In the present study, we screened a gene knock-out library of Rres by RNA slot-blot hybridization and with a genome-wide DNA microarray and identified three Hik-Rre systems, namely, Hik33-Rre31, Hik10-Rre3, and Hik16-Hik41-Rre17, as well as another system that included Rre1, that were involved in perception of salt stress and transduction of the signal. We found that these Hik-Rre systems were identical to those that were involved in perception and transduction of the hyperosmotic stress signal. We compared the induction factors of the salt stress- and hyperosmotic stress- inducible genes that are located downstream of each system and found that these genes responded to the two kinds of stress to different respective extents. In addition, the Hik33-Rre31 system regulated the expression of genes that were specifically induced by hyperosmotic stress, whereas the system that included Rre1 regulated the expression of one or two genes that were specifically induced either by salt stress or by hyperosmotic stress. Our observations suggest that the perception of salt and hyperosmotic stress by the Hik-Rre systems is complex and that salt stress and hyperosmotic stress are perceived as distinct signals by the Hik-Rre systems.