Isolation and partial characterization of Fc gamma-binding proteins of human leukocytes.
Isolation and partial characterization of Fc gamma-binding proteins of human leukocytes.
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人类白细胞 Fc γ 结合蛋白的分离和部分表征。
DOI：
10.1172/jci110410
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Cohen,L
中科院分区：
文献类型：
--
作者：
KulczyckiJr,A;Solanki,L;Cohen,L
我们从人白细胞中分离并部分鉴定了三个Fc结合的大分子。用~(125)I和乳过氧化物酶对正常人和5例慢性淋巴细胞白血病患者的单个核细胞和正常人的中性粒细胞进行了表面放射性标记。细胞洗涤剂增溶后，在不同条件下用重复亲和层析纯化Fc-γ结合大分子，并用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法进行分析。分离的三个放射性标记的大分子保留了与Fc片段结合的特定能力。从正常单核细胞和多形核细胞中分离到一个52,000-64,000摩尔的大分子。43,000-molwt条带是单个核细胞的特征，特别是来自慢性淋巴细胞白血病患者。从正常白细胞中可以获得一个33,000摩尔的重量分子，条件表明它可能是蛋白质水解性片段。
We isolated and partially characterized three Fc-binding macromolecules from human leukocytes. Mononuclear cells from normal individuals and from five patients with chronic lymphocytic leukemia and neutrophils from normal donors were surface radiolabeled by using 125I and lactoperoxidase. After detergent solubilization of the cells, Fc gamma-binding macromolecules were purified by repetitive affinity chromatography under a variety of conditions and analyzed by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. Three radiolabeled macromolecules were isolated that retained specific ability to bind to Fc fragments. A 52,000-64,000-mol wt macromolecule was isolated from normal mononuclear and polymorphonuclear cells. A 43,000-mol wt band was characteristic of mononuclear cells, particularly from patients with chronic lymphocytic leukemia. A 33,000-mol wt molecule could be obtained from normal leukocytes under conditions that suggest it might be a proteolytic fragment.Images