Substances for the initiation of sperm motility in egg-jelly of the Japanese newt, Cynops pyrrhogaster
Substances for the initiation of sperm motility in egg-jelly of the Japanese newt, Cynops pyrrhogaster
复制标题
DOI：
10.2108/zsj.16.793
复制
发表时间：
1999-10-01
影响因子：
0.9
通讯作者：
Onitake, K
中科院分区：
文献类型：
--
作者：
Ukita, M;Itoh, T;Onitake, K
精子活力的启动受某些因素的调节，包括渗透压或离子浓度的变化。启动精子运动的线索是物种所特有的，并适合受精发生的环境。在蝾螈，蝾螈，卵受精在泄殖腔的女性与精子储存在精子库。在这项研究中，我们研究了在这种独特的环境中启动精子活力的可能因素。C.精子渗透压的降低可以启动红腹菌的移动。然而，卵子与干精子受精，并在没有浸入溶液的情况下发育成四细胞期胚胎。受精后，将其置于斯坦伯格盐溶液中，它们继续正常发育至尾芽阶段。这些结果表明，精子运动的启动没有改变精子周围的渗透压。在卵冻提取物中，启动精子运动的活性很强且热稳定，但经蛋白酶处理后消失。ICP发光分析表明，钠、钾、钙和镁离子是鸡蛋冻中的主要阳离子。一价阳离子在高浓度、高pH条件下具有启动精子运动的活性，钙离子的加入使该活性增强。然而，根据这四种离子的浓度和pH值的鸡蛋果冻配制的重构离子溶液没有表现出像鸡蛋果冻提取物那样强的活性。上述结果提示，卵冻中的蛋白质因子和阳离子对精子运动的启动有重要的调节作用。红腹菌
The initiation of sperm motility is regulated by certain factors, including a change of osmolality or ion concentration. The cue for the initiation of sperm motility is unique to species and suits the environment in which the fertilization occurs.In the newt, Cynops pyrrhogaster, eggs are fertilized in the cloaca of the female with sperm stored in the sperm reservoir. In this study, we investigated possible factors initiating sperm motility in this unique environment. Sperm of C. pyrrhogaster could be initiated to move by a decrease of osmolality. However, eggs were fertilized with dry sperm and developed to four-cell stage embryos without immersion in solution. They continued to develop normally to the tail-bud stage when placed in Steinberg's salt solution after fertilization. These results indicate that sperm motility was initiated without the change of osmolality around sperm. In egg-jelly extract, the activity for the initiation of sperm motility was strong and heat stable, but disappeared by proteinase treatment. ICP luminescence analysis revealed that sodium, potassium, calcium and magnesium ions were major cations in egg-jelly. The monovalent cations showed the activity for the initiation of sperm motility in high concentration at high pH, and this activity become stronger by the addition of calcium ion. However, the reconstructed ionic solution that was prepared according to the concentrations of these four ions and pH in egg-jelly did not show the activity as strong as egg-jelly extract. These results suggest that the proteinacious factor and cations in egg-jelly are significant to regulate the initiation of sperm motility in C. pyrrhogaster.