The Arabidopsis CCCH protein C3H14 contributes to basal defense against Botrytis cinerea mainly through the WRKY33-dependent pathway
The Arabidopsis CCCH protein C3H14 contributes to basal defense against Botrytis cinerea mainly through the WRKY33-dependent pathway
复制标题
拟南芥 CCCH 蛋白 C3H14 主要通过 WRKY33 依赖性途径参与针对灰葡萄孢的基础防御
DOI：
10.1111/pce.13771
复制
发表时间：
2020-04-29
影响因子：
7.3
通讯作者：
Chai, Guohua
中科院分区：
文献类型：
--
作者：
Wang, Dian;Xu, Hua;Chai, Guohua
坏死型病原体如灰葡萄孢（Botrytis cinerea）导致显著的作物产量损失。CCCH蛋白在植物抗病反应中起着重要作用。然而，CCCH介导的防御机制对necrotrophic病原体还不清楚。在这里，我们报告，拟南芥CCCH蛋白C3 H14积极调节基础防御B。cinerea主要通过WRKY 33信号转导。C3 H14和C3 H15同时突变导致对B的易感性增强。C3 H14或C3 H15过表达株系表现出降低的易感性。大量的差异表达基因（DEG）中存在的c3 h14 c3 h15双突变体和C3 H14过表达的植物相比，野生型植物在感染后24小时。这些DEG覆盖了B的三分之一以上。灰叶响应WRKY 33靶点，包括参与茉莉酸（JA）/乙烯（ET）信号传导和camalexin生物合成的基因。遗传分析表明，C3 H14主要依赖WRKY 33来调节对B的防御。灰绿色此外，C3 H14激活WRKY 33-ORA 59和-PAD 3级联，以相应地控制JA/ET和camalexin介导的防御反应。而C3 H 14对B是必需的。cinerea诱导的12-氧代-植物二烯酸的产生，并且它还直接介导感染后依赖于ORA 59的JA/ET信号传导。因此，C3 H14可能是WRKY 33介导的防御途径的一种新的转录调节因子。
Necrotrophic pathogens such as Botrytis cinerea cause significant crop yield losses. Plant CCCH proteins play important roles in pathogen resistance responses. However, the CCCH-mediated defense mechanisms against necrotrophic pathogens are unclear. Here, we report that the Arabidopsis CCCH protein C3H14 positively regulates basal defense against B. cinerea mainly by WRKY33 signaling. Simultaneous mutation of C3H14 and its paralog C3H15 resulted in enhanced susceptibility to B. cinerea, while C3H14 or C3H15 overexpression lines exhibited reduced susceptibility. A large number of differentially expressed genes (DEGs) were present in the c3h14c3h15 double mutant and C3H14 overexpression plants compared with wild-type plants at 24 hr post infection. These DEGs covered over one third of B. cinerea-responsive WRKY33 targets, including genes involved in jasmonic acid (JA)/ethylene (ET) signaling, and camalexin biosynthesis. Genetic analysis indicated that C3H14 mainly depended on WRKY33 to modulate defense against B. cinerea. Moreover, C3H14 activated the WRKY33-ORA59 and -PAD3 cascades to correspondingly control JA/ET- and camalexin-mediated defense responses. However, C3H14 was essential for B. cinerea-induced production of 12-oxo-phytodienoic acid and it also directly mediated ORA59-dependent JA/ET signaling after infection. Therefore, C3H14 may act as a novel transcriptional regulator of the WRKY33-mediated defense pathway.