Improved immunoassay for the determination of surfactant protein A (SP-A) in human amniotic fluid.
Improved immunoassay for the determination of surfactant protein A (SP-A) in human amniotic fluid.
复制标题
改进的免疫测定法用于测定人羊水中的表面活性蛋白 A (SP-A)。
DOI：
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发表时间：
1989
影响因子：
2.2
通讯作者：
T. Akino
中科院分区：
文献类型：
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作者：
H. Shimizu;K. Hosoda;M. Mizumoto;Y. Kuroki;H. Sato;K. Kataoka;M. Hagisawa;S. Fujimoto;T. Akino
本文报道了一种简便、快速的羊水表面活性蛋白A（SP-A）免疫测定方法。用单克隆抗体PC 6固定的塑料珠、过氧化物酶标记的单克隆抗体PE 10和在含有0.6%十二烷基硫酸钠/2%Triton X-100的缓冲液中稀释的羊水样品在试管中于45 ℃同时进行免疫反应30分钟。在用含1%Triton X-100的磷酸盐缓冲盐水中的2%脱脂乳洗涤珠后，通过加入底物试剂使过氧化物酶反应显色，并测量吸光度。由于SP-A抗原性的稳定性，因此使用足月时获得的羊水代替纯化的SP-A作为标准。应用该方法对69例妊娠22 ~ 41周羊水中SP-A进行了测定。结果表明，SP-A值可用于预测胎儿肺成熟度。该方法可在1小时内完成检测，可作为临床实验室的常规检测方法。
A simple, improved immunoassay for the determination of human surfactant protein A (SP-A) in human amniotic fluid was developed. The immunoreaction with a monoclonal antibody PC6-immobilized plastic bead, peroxidase-labeled monoclonal antibody PE10 and amniotic fluid sample diluting in the buffer containing 0.6% sodium dodecyl sulfate/2% Triton X-100, was carried out simultaneously at 45 degrees C for 30 min in test tubes. After washing the bead with 2% skimmilk in phosphate buffered saline containing 1% Triton X-100, the peroxidase reaction was developed by adding the substrate reagent and the absorbance was measured. The amniotic fluid obtained at full term was used as a standard instead of purified SP-A, because of the stability of the antigenicity. This immunoassay method was used to measure SP-A in 69 samples of amniotic fluid from 22 to 41 weeks of gestation. The result indicated that the SP-A values obtained by the present immunoassay can be used for predicting the fetal lung maturity. This simplified monoclonal immunoassay completed the measurement within 1 hr, and so it could be used routinely in clinical laboratory.
人肺泡蛋白沉积表面活性剂主要糖蛋白的结构关系。
DOI：
10.1016/0167-4838(87)90070-7
发表时间：
1987
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Ross,GF;Ohning,BL;Tannenbaum,D;Whitsett,JA
通讯作者：
Whitsett,JA