Anti-inflammatory and analgesic activities of the ethanolic extracts from Zanthoxylum riedelianum (Rutaceae) leaves and stem bark
Anti-inflammatory and analgesic activities of the ethanolic extracts from Zanthoxylum riedelianum (Rutaceae) leaves and stem bark
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DOI：
10.1211/jpp.59.8.0014
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发表时间：
2007-08-01
影响因子：
3.3
通讯作者：
Bastos, Jairo Kenupp
中科院分区：
文献类型：
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作者：
Lima, Leonardo Mandalho;Perazzo, Fabio Ferreira;Bastos, Jairo Kenupp
我们评价了花椒（芸香科）叶（LCE）和茎皮（BCE）粗提物的抗炎和镇痛性质。还研究了茎皮提取物的不同级分（己烷，BCEH;二氯甲烷，BCED;乙酸乙酯，BCEE;和冻干含水残留物，BCEW）。我们研究了提取物和馏分的影响，使用大鼠爪水肿试验诱导角叉菜胶，葡聚糖，组胺或制霉菌素;小鼠腹部收缩试验;小鼠热板试验（仅用于LCE和BCE）;和小鼠福尔马林试验。两种提取物和所有BCE组分在角叉菜胶诱导的水肿模型中均显示抗炎活性，但对葡聚糖、组胺或制霉菌素没有。在镇痛模型中，两种提取物均表现出抗伤害性活性，但在中枢性疼痛模型中BCE比LCE更有效。所有BCE组分在腹部收缩试验和福尔马林试验的两个阶段中均显示出显著的抑制作用。当BCED进行植物化学程序时，它导致分离出六种木脂素（芝麻素、甲基雨生芹素、二甲基matairesinol、胡椒醇-4 '-O-γ、γ-二甲基烯丙基醚、kaerophyllin和hinokinin）和三萜（羽扇豆醇）。抑制环氧合酶及其代谢产物可能参与了这种植物的作用机制，考虑到先前的研究报告了所鉴定的木脂素的抗炎和镇痛活性，以及羽扇豆醇的抗炎活性。
We have evaluated the anti-inflammatory and analgesic properties of the leaves (LCE) and stem bark (BCE) crude extracts of Zanthoxylum riedelianum (Rutaceae). Different fractions of the stem bark extract (hexane, BCEH; dichloromethane, BCED; ethyl acetate, BCEE; and lyophilized aqueous residual, BCEW) were also investigated. We studied the effects of the extracts and fractions using the rat paw oedema test induced by carrageenan, dextran, histamine or nystatin; the mouse abdominal constriction test; the mouse hot-plate test (only for LCE and BCE); and the mouse formalin test. Both extracts and all BCE fractions displayed anti-inflammatory activity in the carrageenan-induced oedema model, but not for dextran, histamine or nystatin. Considering the analgesic models, both extracts showed antinociceptive activity, but BCE was more active than LCE in models of central pain. All BCE fractions showed significant inhibition in the abdominal constriction test and in both phases of the formalin test. When BCED was submitted to phytochemical procedures it led to the isolation of six lignans (sesamin, methylpluviatolide, dimethylmatairesinol, piperitol-4'-O-gamma,gamma-dimethylailyl ether, kaerophyllin and hinokinin), and a triterpene (lupeol). Inhibition of cyclooxygenase and its metabolites may have been involved in the mechanism of action of this plant, considering previous studies reporting the anti-inflammatory and analgesic activity for the identified lignans, as well as anti-inflammatory activity for lupeol.