Expression of growth hormone receptors in murine lymphoid cells analyzed by flow cytofluorometry.
Expression of growth hormone receptors in murine lymphoid cells analyzed by flow cytofluorometry.
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通过流式细胞荧光法分析小鼠淋巴细胞中生长激素受体的表达。
DOI：
10.1210/endo.137.5.8612507
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发表时间：
1996
期刊：
影响因子：
4.8
通讯作者：
Mireille Dardenne
中科院分区：
文献类型：
--
作者：
M. Gagnerault;M. Postel;Mireille Dardenne
我们使用流式细胞荧光测定法，使用生物素化的牛 GH 和特定的异硫氰酸荧光素标记的单克隆抗体（定义了不同的淋巴细胞群）分析了小鼠淋巴器官中 GH 受体 (GHR) 的表达。 GHR 在小鼠造血组织以及胎儿、新生儿以及 3 周和 7 周龄动物中广泛表达。在骨髓中，所有造血谱系都表达不同水平的 GH 受体，而在胸腺中，这种表达主要见于 CD4-、CD8-、CD4+CD8+ 和 CD8+ 亚群。在外周，50% 的脾细胞和外周血淋巴细胞以及 20% 的淋巴结细胞呈 GHR 阳性，B 细胞和巨噬细胞（约 50%）上的受体表达比 T 细胞（约 20%）更广泛，其中 CD4+ 和 CD8+ 细胞亚群上均可见标记。有趣的是，用伴刀豆球蛋白 A 或抗 CD3 激活 T 细胞后，携带 GHR 的 CD4+ 和 CD8+ 脾细胞的比例显着增加。最后，我们证明所有表达 GHR 的外周 T 细胞也表达 PRL 受体。我们的研究为研究控制GHR表达的因素以及更好地了解GH在免疫功能调节中的影响提供了分子基础。
We have analyzed the expression of GH receptors (GHR) in murine lymphoid organs using flow cytofluorometry with biotinylated bovine GH and specific fluorescein isothiocyanate-labeled monoclonal antibodies defining distinct lymphoid cell populations. GHR were widely expressed in al murine hematopoietic tissues and in fetuses, newborns, and 3- and 7-week-old animals. In the bone marrow, all hematopoietic lineages expressed variable levels of GH receptors, whereas in the thymus, this expression was mainly seen in CD4-, CD8-, CD4+CD8+, and CD8+ subpopulations. At the periphery, 50% of splenocytes and peripheral blood lymphocytes and 20% of lymph node cells were GHR positive, with a wider receptor expression on B cells and macrophages (approximately 50%) than on T cells (approximately 20%), where the labeling was seen on both CD4+ and CD8+ cell subsets. Interestingly, the proportion of GHR-bearing CD4+ and CD8+ splenocytes significantly increased after T cell activation with Concanavalin A or anti-CD3. Finally, we demonstrated that all peripheral T cells expressing GHR also expressed PRL receptors. Our study provides a molecular basis to study the factors controlling GHR expression and to better understand the influence of GH in the regulation of immune function.