A GENETIC-ANALYSIS OF TYPE-2 (NON-INSULIN-DEPENDENT) DIABETES-MELLITUS IN PUNJABI SIKHS AND BRITISH CAUCASOID PATIENTS
A GENETIC-ANALYSIS OF TYPE-2 (NON-INSULIN-DEPENDENT) DIABETES-MELLITUS IN PUNJABI SIKHS AND BRITISH CAUCASOID PATIENTS
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DOI：
10.1111/j.1464-5491.1987.tb00923.x
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发表时间：
1987-11-01
影响因子：
3.5
通讯作者：
MATHER, H
中科院分区：
文献类型：
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作者：
HITMAN, GA;KARIR, PK;MATHER, H
使用胰岛素受体、胰岛素和HLA-D α链基因探针对糖尿病和非糖尿病旁遮普锡克人（n= 164）进行了非胰岛素依赖型糖尿病标志物的遗传分析。此外，英国高加索人（n=163）进行了研究，使用胰岛素受体探针。胰岛素受体基因限制性片段长度多态性的定义，使用Southern印迹技术和限制性内切酶Bgl II和BamHI。在旁遮普锡克教徒和英国高加索人中，限制性片段长度多态性均不能区分非胰岛素依赖型糖尿病受试者和对照组。在锡克教徒中，未发现胰岛素基因高变区或HLA-DR/DQ/DXα链限制性片段长度多态性与非胰岛素依赖型糖尿病相关。因此，我们得出结论，尽管亚洲人非胰岛素依赖型糖尿病的患病率很高，但我们无法使用可用的克隆基因探针找到这种疾病的任何遗传标记。
A genetic analysis of diabetic and non‐diabetic Punjabi Sikhs (n= 164) was made for markers of non‐insulin‐dependent diabetes mellitus using insulin receptor, insulin, and HLA‐D αchain gene probes. Additionally British Caucasoids (n=163) were studied using the insulin receptor probe. Insulin receptor gene restriction fragment length polymorphisms were defined using Southern blot techniques and the restriction enzyme Bgl II and Bam HI. In Punjabi Sikhs and British Caucasoids neither of the restriction fragment length polymorphisms distinguished non‐insulin‐dependent diabetes mellitus subjects from controls. In the Sikhs no association with non‐insulin‐dependent diabetes mellitus was seen with the hypervariable region of the insulin gene or with HLA‐DR/DQ/DXα chain restriction fragment length polymorphisms. We therefore conclude that despite the high prevalence of non‐insulin‐dependent diabetes mellitus in Asians we were unable to find any genetic markers for this disease using the available cloned gene probes.