Analytical selenoamino acid studies by chromatography with interfaced atomic mass spectrometry and atomic emission spectral detection
Analytical selenoamino acid studies by chromatography with interfaced atomic mass spectrometry and atomic emission spectral detection
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通过色谱结合接口原子质谱和原子发射光谱检测进行分析硒氨基酸
DOI：
10.1007/s002160051105
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
E. Denoyer
中科院分区：
文献类型：
--
作者：
P. Uden;S. Bird;M. Kotrebai;Paula B Nolibos;J. Tyson;E. Block;E. Denoyer
据报道，食用富硒植物或基于酵母的营养补充剂可提供抗癌益处，这些益处依赖于硒化合物。这些硒化合物的分离和鉴定对于了解其活性至关重要。植物和酵母将土壤或生长介质中的无机硒转化为有机硒化合物，可能遵循类似于硫同化途径的途径。产生的非挥发性硒化合物包括硒代氨基酸，其中一些已显示抗癌活性。本文提出了一种离子对色谱-电感耦合等离子体质谱法分离真实的样品中可能存在的硒代氨基酸标准品的方法。该方法允许分离硒代氨基酸，包括诸如Se-1-丙烯基-dl-硒代半胱氨酸的顺反异构体的分析物。该方法还提供了确定硒氧化物和可能的硒酮的存在的能力，以及通过选择性衍生跟踪其他功能和反应的能力。或者，硒代氨基酸用氯甲酸乙酯处理以产生稳定的挥发性衍生物，其适合于用元素特异性原子发射检测（GC-AED）进行GC分离。结果总硒测定和形态的富硒酵母为基础的营养补充剂，富硒葱属蔬菜和生物修复样品。
Consumption of selenium enriched plants or yeast-based nutritional supplements has been reported to provide anticarcinogenic benefits which are selenium compound dependent. Separation and identification of these selenium compounds is critical to understand the activity. Plants and yeast convert inorganic selenium in the soil or growth media into organoselenium compounds, probably following a route similar to the sulfur assimilatory pathway. Non-volatile selenium compounds produced include selenoamino acids, some of which have shown anticarcinogenic activity. Volatile compounds produced by chemical reaction of involatile precursors have also been found. An ion pair chromatographic method with ICP-MS detection for the separation of selenoamino acid standards potentially present in real samples is given. The method allows separation of selenoamino acids including such analytes as the cis-trans isomers of Se-1-propenyl-dl-selenocysteine. The method also provides the capability of determining the presence of selenoxides and possibly selenones, and tracking of other functionalities and reactions by selective derivatization. Alternatively, selenoamino acids are treated with ethylchloroformate to produce stable volatile derivatives which are amenable to GC separation with element specific atomic emission detection (GC-AED). Results of total selenium determination and speciation of selenium enriched yeast-based nutritional supplements, selenium enriched allium vegetables and bioremediation samples are presented.