Expression of CD180, a toll-like receptor homologue, is up-regulated in children with Kawasaki disease
Expression of CD180, a toll-like receptor homologue, is up-regulated in children with Kawasaki disease
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DOI：
10.1007/s00109-005-0010-8
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发表时间：
2006-02-01
影响因子：
4.7
通讯作者：
Ishii, E
中科院分区：
文献类型：
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作者：
Imayoshi, M;Yamamoto, S;Ishii, E
川崎病（Kawasaki disease， KD）是一种以冠状动脉动脉瘤为特征的儿童急性发热性疾病。由于该病流行频次大，家族性发病率高，因此认为该病是由感染因子引起的。由于KID患儿外周血B细胞水平升高和功能障碍，我们研究了KD患儿和细菌或病毒感染患儿B细胞中toll样受体同源物cd180的表达。cd180阳性B细胞在KID或病毒感染儿童中的百分比明显高于细菌感染儿童或健康对照。用患者与健康对照的平均荧光强度比比较CD180的表达水平时，发现KD或病毒感染儿童的CD180表达水平也显著上调。为了阐明病毒感染对cd180表达的影响，我们用合成的双链RNA poly - inosic -cytidyric [poly(IC)]刺激B细胞。Poly（IC）在体外B细胞中明显增强CD180蛋白和信使RNA水平的表达。这些结果表明，类似的机制可能参与了KD或病毒感染患者B细胞cd180表达的上调。
Kawasaki disease (KD) is an acute febrile illness in childhood characterized by the fort-nation of aneurysms in coronary arteries. It is believed that KD is caused by infectious agents because of its epidemic waves and high incidence of familial occurrence. Because an increase in the levels and dysfunction of B cells in peripheral blood was reported in KID, we investigated the expression of cluster of differentiation 180 (CD 180), a toll-like receptor homologue, in the B cells of children with KD, and in those with bacterial or viral infections. The percentages of CD 180 positive B cells were significantly higher in children with KID or viral infections than in those with bacterial infections or in healthy controls. When the expression levels of CD 180 were compared by using the mean fluorescent intensity ratio of patients to healthy controls, the level of CD180 expression was also significantly up-regulated in children with KD or viral infections. To clarify the effect of viral infection on the expression of CD 180, B cells were stimulated with poly inosinic-cytidyric acid [poly(IC)], a synthetic double-stranded RNA. Poly(IC) clearly enhance CD180 expression in B cells in vitro, both at the protein and messenger RNA levels. These results suggest that similar mechanisms may be involved in the up-regulation of B cell CD 180 expression in patients with either KD or viral infections.