Diterpene cyclases responsible for the biosynthesis of phytoalexins, momilactones A, B, and oryzalexins A-F in rice
Diterpene cyclases responsible for the biosynthesis of phytoalexins, momilactones A, B, and oryzalexins A-F in rice
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水稻中负责植物缬草素、莫米内酯 A、B 和oryzalexins A-F 生物合成的二萜环化酶
DOI：
10.1271/bbb.68.2001
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发表时间：
2004-09-01
影响因子：
1.6
通讯作者：
Toyomasu, T
中科院分区：
文献类型：
--
作者：
Otomo, K;Kanno, Y;Toyomasu, T
水稻（Oryza sativa L.）产生二萜植物抗毒素，例如momilactones、oryzalexins和phytocassane。利用水稻基因组信息和重组酶的体外分析，我们鉴定了编码A型二萜环化酶9 β-pimara-7，15-diene synthase和ent-sandaracopimara-diene synthase的基因（OsKS 4和OsKS 10），它们分别参与momilactones A，B和oryzalexins A-F的生物合成。这两个基因的转录水平显着增加后，紫外线（UV）处理，这是一致的，通过UV的植物抗毒素的产量增加。这两个基因可能是理解水稻植物防御机制的有力工具。
Rice (Oryza sativa L.) produces diterpene phytoalexins, such as momilactones, oryzalexins, and phytocassanes. Using rice genome information and in vitro assay with recombinant enzymes, we identified genes (OsKS4 and OsKS10) encoding the type-A diterpene cyclases 9beta-pimara-7,15-diene synthase and ent-sandaracopimara-diene synthase which are involved in the biosynthesis of momilactones A, B and oryzalexins A-F respectively. Transcript levels of these two genes increased remarkably after ultraviolet (UV) treatment, which is consistent with elevated production of phytoalexins by UV. These two genes might prove powerful tools for understanding plant defense mechanisms in rice.