The effect of factor X level on thrombin generation and the procoagulant effect of activated factor VII in a cell-based model of coagulation.
The effect of factor X level on thrombin generation and the procoagulant effect of activated factor VII in a cell-based model of coagulation.
复制标题
在基于细胞的凝血模型中，因子 X 水平对凝血酶生成的影响以及活化因子 VII 的促凝血作用。
DOI：
10.1097/00001721-200004001-00002
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Hoffman,M
中科院分区：
文献类型：
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作者：
Allen,GA;Monroe3rd,DM;Roberts,HR;Hoffman,M
我们使用基于细胞的正常止血和血友病体外模型来解决因子 (F) X 浓度是否影响对高剂量活化因子 VII (FVIIa) 的止血反应的问题。在模拟正常组织因子引发的体内止血的条件下，我们发现只需要极少量的 FX（相当于正常血浆水平的约 3%）即可支持“正常”水平的凝血酶生成。这表明，在体内正常条件下，FX水平并不显着影响止血功能。相比之下，在模拟血友病状况的实验中，FX 水平对高剂量 FVIIa 存在下产生的凝血酶水平有显着影响。这一发现表明 FX 的血浆水平可能影响血友病患者对高剂量 FVIIa 治疗的止血反应。血液凝固纤维蛋白溶解11（补充1）：S3-S7© 2000 Lippincott Williams & Wilkins。
We used a cell-based, in-vitro model of normal hemostasis and hemophilia to address the question of whether factor (F) X concentration affects the hemostatic response to high-dose activated factor VII (FVIIa). Under conditions designed to mimic normal tissue factorinitiated hemostasisin vivo, we found that only a very small amount of FX-equivalent to about 3% of the normal plasma level-was required to support a ‘normal’level of thrombin generation. This suggests that, under normal conditionsin vivo, the level of FX does not significantly affect hemostatic function. By contrast, in experiments designed to mimic the hemophilic condition, the level of FX had a significant effect on the level of thrombin generated in the presence of high-dose FVIIa. This finding suggests that the plasma level of FX could affect the hemostatic response of hemophilic patients to high-dose FVIIa therapy. Blood Coagul Fibrinolysis11 (suppl 1): S3-S7© 2000 Lippincott Williams & Wilkins.