Six-Pulse RIDME Sequence to Avoid Background Artifacts
Six-Pulse RIDME Sequence to Avoid Background Artifacts
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DOI：
10.1007/s00723-021-01326-1
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发表时间：
2021-04-09
影响因子：
1
通讯作者：
Schiemann, Olav
中科院分区：
文献类型：
--
作者：
Abdullin, Dinar;Suchatzki, Miriam;Schiemann, Olav
弛豫诱导的偶极调制增强（RIDME）是测量电子自旋中心之间纳米尺度距离的一种有价值的方法。这种距离在结构生物学中被广泛用于研究生物分子结构和跟踪其构象变化。尽管近年来RIDME技术有了很大的进步，但对RIDME初级信号的背景分析仍然具有挑战性。特别是，最近有研究表明，五脉冲RIDME信号包含一个伪影，可能会阻碍RIDME时间迹的距离分布的准确提取[Ritsch等人报道]（Phys Chem Chem Phys 21: 9810, 2019）。在这里，这个人工制品，以及另外一个确定的人工制品，在几个模型化合物上进行了系统的研究，并讨论了这两个人工制品的可能起源。此外，提出了一种新的六脉冲RIDME序列，消除了对提取的距离分布影响最大的伪影。算例验证了该脉冲序列的有效性。
Relaxation induced dipolar modulation enhancement (RIDME) is a valuable method for measuring nanometer-scale distances between electron spin centers. Such distances are widely used in structural biology to study biomolecular structures and track their conformational changes. Despite significant improvements of RIDME in recent years, the background analysis of primary RIDME signals remains to be challenging. In particular, it was recently shown that the five-pulse RIDME signals contain an artifact which can hinder the accurate extraction of distance distributions from RIDME time traces [as reported by Ritsch et al. (Phys Chem Chem Phys 21: 9810, 2019)]. Here, this artifact, as well as one additionally identified artifact, are systematically studied on several model compounds and the possible origins of both artifacts are discussed. In addition, a new six-pulse RIDME sequence is proposed that eliminates the artifact with the biggest impact on the extracted distance distributions. The efficiency of this pulse sequence is confirmed on several examples.