Heme b in marine phytoplankton and particulate material from the North Atlantic Ocean
Heme b in marine phytoplankton and particulate material from the North Atlantic Ocean
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DOI：
10.3354/meps10367
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发表时间：
2013-01-01
影响因子：
2.5
通讯作者：
Achterberg, Eric P.
中科院分区：
文献类型：
--
作者：
Honey, David J.;Gledhill, Martha;Achterberg, Eric P.
本文测定了6种海洋浮游植物（杜氏杜氏藻（Dunaliellatertiolecta）、赫氏埃米利亚藻（Emilianiahuxleyi）、威氏海链藻（Thalassiosiraweissfloprotein）、海链藻（T. oceanica、三角褐指藻（Phaeodactylum tricor nutum）和聚球藻（Synechococcus sp.WH7803）。同时还检测了血红素B对弱光和硝酸盐胁迫的响应。huxleyi和T.海洋。从实验室培养的结果进行了比较，血红素B确定在北大西洋的颗粒材料。在培养物中，血红素B占总铁库的18 +/- 14%（SE）。铁和硝酸盐浓度的降低导致细胞内血红素B浓度的降低，以每摩尔碳表示。叶绿素a（chl a）与血红素B b的比值在E. huxleyi和D. tertiolecta在响应有限的光和养分供应增加，但略有下降或没有变化的硅藻和蓝藻聚球藻。WH 7803。血红素B：颗粒有机碳（POC）和叶绿素a：血红素B的比例在北大西洋的浮游植物培养中观察到的范围内。在表面混合层中，血红素B：POC比例的减少与营养盐浓度的减少有关。叶绿素a：血红素B的比例随深度增加，因此主要受光的可用性。北大西洋的血红素B、叶绿素a和POC之间的相对关系可能代表了细胞根据环境光和营养条件进行由叶绿素a（光捕获）和血红素B（例如电子传递）驱动的细胞过程的能力的变化。
Concentrations of heme b, the ironcontaining prosthetic group of many hemoproteins, were measured in 6 species of marine phytoplankton (Dunaliella tertiolecta, Emiliania huxleyi, Thalassio sira weissflogii, T. oceanica, Phaeodactylum tricor nutum and Synechococcus sp. WH7803) that were subjected to variations in iron concentration. Changes in heme b in response to reduced light and nitrate were also ex amined for E. huxleyi and T. oceanica. Results from laboratory cultures were compared with heme b determined in particulate material in the North Atlantic. In cultures, heme b made up 18 +/- 14% (SE) of the total iron pool. Reduced iron and nitrate concentrations resulted in a decreased intracellular heme b concentration, expressed as per mole carbon. Chlorophyll a (chl a) to heme b ratios in E. huxleyi and D. tertiolecta in creased in response to limited light and nutrient availability, but slightly decreased or did not change in the diatoms and the cyanophyte Synechococcus sp. WH7803. The heme b: particulate organic carbon (POC) and chl a: heme b ratios in the North Atlantic were within the range observed in phytoplankton cultures. In the surface mixed layer, decreases in heme b: POC ratios were linked to decreases in nutrient concentrations. Chl a: heme b ratios increased with depth and were thus primarily affected by light availability. Relative relationships between heme b, chl a and POC in the North Atlantic likely represented a change in the ability of cells to undertake cellular processes driven by chl a (light harvesting) and heme b (e.g. electron transport) according to ambient light and nutrient conditions.