NUCLEOTIDE-SEQUENCE AND GENETIC-ANALYSIS OF A 13.1-KILOBASE-PAIR PSEUDOMONAS-DENITRIFICANS DNA FRAGMENT CONTAINING 5 COB GENES AND IDENTIFICATION OF STRUCTURAL GENES ENCODING COB(I)ALAMIN ADENOSYLTRANSFERASE, COBYRIC ACID SYNTHASE, AND BIFUNCTIONAL COBINAMIDE KINASE-COBINAMIDE PHOSPHATE GUANYLYLTRANSFERASE
NUCLEOTIDE-SEQUENCE AND GENETIC-ANALYSIS OF A 13.1-KILOBASE-PAIR PSEUDOMONAS-DENITRIFICANS DNA FRAGMENT CONTAINING 5 COB GENES AND IDENTIFICATION OF STRUCTURAL GENES ENCODING COB(I)ALAMIN ADENOSYLTRANSFERASE, COBYRIC ACID SYNTHASE, AND BIFUNCTIONAL COBINAMIDE KINASE-COBINAMIDE PHOSPHATE GUANYLYLTRANSFERASE
复制标题
DOI：
10.1128/jb.173.19.6074-6087.1991
复制
发表时间：
1991-10-01
影响因子：
3.2
通讯作者：
THIBAUT, D
中科院分区：
文献类型：
--
作者：
CROUZET, J;LEVYSCHIL, S;THIBAUT, D
一个13.1kb的DNA片段携带假单胞菌cob基因已被测序。 核苷酸序列和遗传分析表明，该片段包含5个不同的cob基因，命名为cobN到cobQ和cobW。 基于纯化的Cob蛋白的NH 2-末端序列和分子量的相似性，CobQ被鉴定为Cobyric acid synthase，CobP被鉴定为同时具有Cobinamide激酶和Cobinamide磷酸鸟苷酰转移酶活性的双功能酶，CobO被鉴定为cob（I）alamin腺苷转移酶。 CobN被认为在钴插入反应中起作用。 在13.1-kb片段上鉴定了另外四个开放阅读框，但它们的染色体失活并不导致钴胺素阴性表型。
A 13.1-kb DNA fragment carrying Pseudomonas denitrificans cob genes has been sequenced. The nucleotide sequence and genetic analysis revealed that this fragment contained five different cob genes named cobN to cobQ and cobW. Based on the similarity of NH2-terminal sequences and molecular weights of the purified Cob proteins, CobQ was identified as cobyric acid synthase, CobP was identified as a bifunctional enzyme exhibiting both cobinamide kinase and cobinamide phosphate guanylyltransferase activities, and CobO was identified as cob(I)alamin adenosyltransferase. CobN is proposed to play a role in cobalt insertion reactions. Four other open reading frames were identified on the 13.1-kb fragment, but their chromosomal inactivation did not lead to a cobalamin-minus phenotype.