Effect of surface properties of silica nanoparticles on their cytotoxicity and cellular distribution in murine macrophages.
Effect of surface properties of silica nanoparticles on their cytotoxicity and cellular distribution in murine macrophages.
复制标题
二氧化硅纳米颗粒的表面特性对其细胞毒性和在小鼠巨噬细胞中的细胞分布的影响。
DOI：
10.1186/1556-276x-6-93
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发表时间：
2011-01-18
影响因子：
--
通讯作者：
Tsutsumi Y
中科院分区：
文献类型：
--
作者：
Nabeshi H;Yoshikawa T;Arimori A;Yoshida T;Tochigi S;Hirai T;Akase T;Nagano K;Abe Y;Kamada H;Tsunoda S;Itoh N;Yoshioka Y;Tsutsumi Y
表面性质通常被认为是开发更安全形式的纳米材料（NM）的重要因素。然而，从研究细胞对NM的反应中获得的结果往往是矛盾的。因此，本研究的目的是研究二氧化硅纳米颗粒的表面性质和它们对小鼠巨噬细胞系（RAW 264.7）的细胞毒性之间的关系。二氧化硅纳米颗粒的表面是未改性的（nSP 70）或用胺（nSP 70-N）或羧基（nSP 70-C）改性的。首先，对二氧化硅纳米粒子的性质进行了表征。然后将RAW264.7细胞暴露于nSP 70、nSP 70-N或nSP 70-C，并通过分析DNA合成来监测任何细胞毒性作用。本研究的结果表明，与未修饰的nSP 70相比，nSP 70-N和nSP 70-C对DNA合成活性的影响较小。对二氧化硅纳米颗粒的细胞内定位的分析显示，nSP 70已经渗透到细胞核中，而nSP 70-N和nSP 70-C没有显示出细胞核定位。这些结果表明，细胞内定位是这些二氧化硅纳米颗粒的细胞毒性的关键因素。因此，二氧化硅纳米颗粒的表面性质在决定其安全性方面起着重要作用。我们的研究结果表明，优化二氧化硅纳米粒子的表面特性将有助于开发更安全的纳米材料。
Surface properties are often hypothesized to be important factors in the development of safer forms of nanomaterials (NMs). However, the results obtained from studying the cellular responses to NMs are often contradictory. Hence, the aim of this study was to investigate the relationship between the surface properties of silica nanoparticles and their cytotoxicity against a murine macrophage cell line (RAW264.7). The surface of the silica nanoparticles was either unmodified (nSP70) or modified with amine (nSP70-N) or carboxyl groups (nSP70-C). First, the properties of the silica nanoparticles were characterized. RAW264.7 cells were then exposed to nSP70, nSP70-N, or nSP70-C, and any cytotoxic effects were monitored by analyzing DNA synthesis. The results of this study show that nSP70-N and nSP70-C have a smaller effect on DNA synthesis activity by comparison to unmodified nSP70. Analysis of the intracellular localization of the silica nanoparticles revealed that nSP70 had penetrated into the nucleus, whereas nSP70-N and nSP70-C showed no nuclear localization. These results suggest that intracellular localization is a critical factor underlying the cytotoxicity of these silica nanoparticles. Thus, the surface properties of silica nanoparticles play an important role in determining their safety. Our results suggest that optimization of the surface characteristics of silica nanoparticles will contribute to the development of safer forms of NMs.