SUPERMAN, a regulator of floral homeotic genes in Arabidopsis.
SUPERMAN, a regulator of floral homeotic genes in Arabidopsis.
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发表时间：
1992-03
期刊：
影响因子：
4.6
通讯作者：
J. Bowman;H. Sakai;T. Jack;D. Weigel;U. Mayer;E. Meyerowitz
中科院分区：
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作者：
J. Bowman;H. Sakai;T. Jack;D. Weigel;U. Mayer;E. Meyerowitz
我们描述了一个座位，超人，在这种突变中，导致额外的雄蕊发育，以牺牲拟南芥花的中心心皮为代价。用扫描电子显微镜描述了超人花从最初的原基到成熟花的发育过程。还描述了用超人等位基因构建的双重和三重突变菌株的发展，以及先前发现的导致花器官特性改变的同源异型突变。基本上，在超人无配偶和超人无花瓣2双突变体中观察到了加性表型。无瓣瓣或雌蕊与超人等位基因之间的上位性关系表明，超人基因产物可能是这些花的同源异型基因的调节因子。为了验证这一点，我们研究了AGAMOUS和APETALA3在超人花中的表达模式。在野生型花中，APETALA3的表达仅限于花瓣和雄蕊指定所需的第二轮和第三轮。相比之下，在超人花中，APETALA3的表达扩大到包括大多数正常情况下构成第四轮的细胞。这种异源APETALA3的表达可能是超人花额外雄蕊发育的原因之一。与野生型花相比，超人花中AGAMOUS表达的空间模式保持不变。综上所述，这些数据表明，野生型超人基因产物的功能之一是负调控花第四轮的APETALA3。此外，超人突变体表现出花分生组织的确定性丧失，这种影响似乎是由APETALA3和雌蕊基因产物介导的。
We describe a locus, SUPERMAN, mutations in which result in extra stamens developing at the expense of the central carpels in the Arabidopsis thaliana flower. The development of superman flowers, from initial primordium to mature flower, is described by scanning electron microscopy. The development of doubly and triply mutant strains, constructed with superman alleles and previously identified homeotic mutations that cause alterations in floral organ identity, is also described. Essentially additive phenotypes are observed in superman agamous and superman apetala2 double mutants. The epistatic relationships observed between either apetala3 or pistillata and superman alleles suggest that the SUPERMAN gene product could be a regulator of these floral homeotic genes. To test this, the expression patterns of AGAMOUS and APETALA3 were examined in superman flowers. In wild-type flowers, APETALA3 expression is restricted to the second and third whorls where it is required for the specification of petals and stamens. In contrast, in superman flowers, APETALA3 expression expands to include most of the cells that would normally constitute the fourth whorl. This ectopic APETALA3 expression is proposed to be one of the causes of the development of the extra stamens in superman flowers. The spatial pattern of AGAMOUS expression remains unaltered in superman flowers as compared to wild-type flowers. Taken together these data indicate that one of the functions of the wild-type SUPERMAN gene product is to negatively regulate APETALA3 in the fourth whorl of the flower. In addition, superman mutants exhibit a loss of determinacy of the floral meristem, an effect that appears to be mediated by the APETALA3 and PISTILLATA gene products.