14-3-3 protein detection in the cerebrospinal fluid of patients with influenza-associated encephalopathy
14-3-3 protein detection in the cerebrospinal fluid of patients with influenza-associated encephalopathy
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DOI：
10.1177/08830738060210070502
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发表时间：
2006-07-01
影响因子：
1.9
通讯作者：
Kohno, Yoichi
中科院分区：
文献类型：
--
作者：
Fujii, Katsunori;Tanabe, Yuzo;Kohno, Yoichi
流感相关脑病的特点是儿童感染流感后出现高热、抽搐和意识丧失，但其病理生理学仍有待阐明。我们通过免疫印迹法检测了 4 名流感相关脑病患者、4 名无脑病的流感患者和 4 名另一种脑病患者的脑脊液中的 14-3-3 蛋白（酸性脑蛋白）。有趣的是，我们在所有四名流感相关脑病患者（100%）中检测到了 14-3-3 蛋白，但在其他任何患者中均未检测到。在流感相关脑病患者的脑脊液中发现了 14-3-3 isofonus，包括 beta、gamma、epsilon、xi 和 0，表明大脑受到广泛损害。我们的结论是，脑脊液中的 14-3-3 蛋白在流感相关脑病中高度可检测，因此可用作快速诊断标记物。
Influenza-associated encephalopathy is characterized by high fever, convulsions, and loss of consciousness associated with influenza infection in children, but its pathophysiology remains to be clarified. We examined 14-3-3 proteins, which are acidic brain proteins, in cerebrospinal fluid by immunoblotting in four patients with influenza-associated encephalopathy, four patients with influenza without encephalopathy, and four patients with another encephalopathy. Interestingly, we detected 14-3-3 proteins in all four patients with influenza-associated encephalopathy (100%) but not in any of the other patients. 14-3-3 isofonus, including beta, gamma, epsilon, xi, and 0, were found in the cerebrospinal fluid of the patients with influenza-associated encephalopathy, suggesting extensive damage to the brain. We conclude that 14-3-3 proteins in cerebrospinal fluid are highly detectable in influenza-associated encephalopathy and thus can be used as a rapid diagnostic marker.