Vascularization-The Conduit to Viable Engineered Tissues
Vascularization-The Conduit to Viable Engineered Tissues
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DOI：
10.1089/ten.teb.2008.0193
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发表时间：
2009-06-01
影响因子：
6.4
通讯作者：
Levenberg, Shulamit
中科院分区：
文献类型：
--
作者：
Kaully, Tamar;Kaufman-Francis, Keren;Levenberg, Shulamit
厚的三维工程化组织构建体的长期活性是一项重大挑战。要解决这一问题，需要开发类血管网络，由于植入后血管化改善，这将使构建体在体外存活并在体内整合。由多个研究小组的工作产生了几种旨在实现工程化组织血管化的方法：（1）在聚合物支架中包含血管生成生长因子以实现长效释放，（2）内皮细胞与靶组织细胞和血管生成信号细胞共培养，（3）使用微制造方法创建设计的通道，以允许营养物质流动和/或引导内皮细胞附着，以及（4）对器官和血管进行脱细胞处理以创建细胞外基质。正如一些最新研究已经证明的那样，将这些方法中的几种结合起来有望产生协同效应。本文综述了每种方法的进展以及工程化组织血管化的近期成果。
Long-term viability of thick three-dimensional engineered tissue constructs is a major challenge. Addressing it requires development of vessel-like network that will allow the survival of the construct in vitro and its integration in vivo owing to improved vascularization after implantation. Resulting from work of various research groups, several approaches were developed aiming engineered tissue vascularization: (1) embodiment of angiogenesis growth factors in the polymeric scaffolds for prolonged release, (2) coculture of endothelial cells with target tissue cells and angiogenesis signaling cells, (3) use of microfabrication methods for creating designed channels for allowing nutrients to flow and/or for directing endothelial cells attachment, and (4) decellularization of organs and blood vessels for creating extracellular matrix. A synergistic effect is expected by combining several of these approaches as already demonstrated in some of the latest studies. Current paper reviews the progress in each approach and recent achievements toward vascularization of engineered tissues.