Impact of the transcriptional regulator, Ace2, on the Candida glabrata secretome.
Impact of the transcriptional regulator, Ace2, on the Candida glabrata secretome.
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转录调节因子 Ace2 对光滑念珠菌分泌组的影响。
DOI：
10.1002/pmic.200800706
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发表时间：
2010
期刊：
影响因子：
3.4
通讯作者：
Stead DA
中科院分区：
文献类型：
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作者：
Stead DA
光滑念珠菌是人类的主要致病真菌，其毒力较弱。glabratais增加的转录因子，Ace 2的失活。我们先前对Ace 2失活对细胞内蛋白质组影响的研究表明，C. glabrata ace 2突变体可能是由分泌组的差异引起的。因此，在这项研究中，我们已经确定了C。glabratasecretome和检查Ace 2失活后，这种细胞外蛋白质组的影响。我们已经在野生型C的分泌组中鉴定了31种不同的蛋白质。通过MS/MS分析从生长培养基中沉淀并通过亲和层析富集刀豆球蛋白A的蛋白质。这些蛋白质中的大多数被预测为细胞壁蛋白质、细胞壁修饰酶和胆甾醇蛋白酶。C.未检测到内切几丁质酶Cts 1和内切葡聚糖酶Egt 2。Glabratasecretome后Ace 2失活。这可以解释C.光滑果ace 2细胞。Ace 2的失活也导致在C.切除器这些蛋白质的释放可能与face 2细胞的高毒力有关。
Candida glabratais a major fungal pathogen of humans, and the virulence ofC. glabratais increased by inactivation of the transcription factor, Ace2. Our previous examination of the effects of Ace2 inactivation upon the intracellular proteome suggested that the hypervirulence ofC. glabrata ace2mutants might be caused by differences in the secretome. Therefore in this study we have characterised theC. glabratasecretome and examined the effects of Ace2 inactivation upon this extracellular proteome. We have identified 31 distinct proteins in the secretome of wild‐typeC. glabratacells by MS/MS of proteins that were precipitated from the growth medium and enriched by affinity chromatography on concanavalin A. Most of these proteins are predicted to be cell wall proteins, cell wall modifying enzymes and aspartyl proteinases. The endochitinase Cts1 and the endoglucanase Egt2 were not detected in theC. glabratasecretome following Ace2 inactivation. This can account for the cell separation defect ofC. glabrata ace2cells. Ace2 inactivation also resulted in the detection of new proteins in theC. glabratasecretome. The release of such proteins might contribute to the hypervirulence oface2cells.