Quantification of Fusarium graminearum in harvested grain by real-time polymerase chain reaction to assess efficacies of fungicides on fusarium head blight, deoxynivalenol contamination, and yield of winter wheat.
Quantification of Fusarium graminearum in harvested grain by real-time polymerase chain reaction to assess efficacies of fungicides on fusarium head blight, deoxynivalenol contamination, and yield of winter wheat.
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DOI：
10.1094/phyto-99-1-0095
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发表时间：
2009
期刊：
影响因子：
3.2
通讯作者：
Yu Zhang;P. Fan;X. Zhang;Changjun Chen;Mingguo Zhou
中科院分区：
文献类型：
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作者：
Yu Zhang;P. Fan;X. Zhang;Changjun Chen;Mingguo Zhou
采用基于实时聚合酶链反应的方法和目视病害评价方法，评价了Js399-19、戊唑唑、戊唑唑与噻美唑的混合物、嘧菌酯、多菌灵和噻美唑对小麦赤霉病（FHB）和脱氧雪腐镰刀菌醇（DON）污染的防治效果以及对冬小麦产量的影响。Nannong没有。9918)田间条件下人工接种谷草镰刀菌后。测定了2006年和2007年稻瘟病小穗发病率（IIS）、稻瘟病菌DNA （Tri5 DNA）含量、总DON（含DON、3-乙酰脱氧雪腐腐菌醇和15-乙酰脱氧雪腐腐菌醇）浓度和千粒重（TGW）。IIS和Log10Tri5 DNA与收获籽粒总DON浓度呈显著正相关。Js399-19、戊康唑和戊康唑与噻美姆的混合处理显著降低了籽粒内FHB的IIS、Tri5 DNA的数量和总DON，增加了总重。虽然氮嘧菌酯、多菌灵和硫胺可以增加TGW，但与未处理的对照相比，它们对禾谷F. graminearum的发生没有影响。令人惊讶的是，氮嘧菌酯和多菌灵显著增加了收获谷物中总DON含量，因为它们可能刺激了每个Tri5 DNA的总DON产量。杀菌剂Js399-19、戊康唑和戊康唑与噻美姆复配对小麦赤霉病的防治效果最好，对减少DON污染效果最好。
We used a real time polymerase chain reaction-based assay and visual disease assessment to evaluate the efficacies of Js399-19, tebuconazole, a mixture of tebuconazole and thiram, azoxystrobin, carbendazim, and thiram on the development of Fusarium head blight (FHB) and deoxynivalenol (DON) contamination and on the yield of winter wheat (cv. Nannong no. 9918) after artificial inoculation under field conditions with Fusarium graminearum. The incidence of infected spikelets (IIS), amount of F. graminearum DNA (Tri5 DNA), total DON (containing DON, 3-acetyl-deoxynivalenol, and 15-acetyl-deoxynivalenol) concentration, and 1,000-grain weight (TGW) were quantified in 2006 and 2007. A strong positive correlation was found between IIS or Log10Tri5 DNA and total DON concentration in the harvested grain. The Js399-19, tebuconazole, and the mixture of tebuconazole and thiram significantly reduced IIS of FHB, amount of Tri5 DNA, and total DON within the grain and increased TGW. Although azoxystrobin, carbendazim, and thiram can increase TGW, they had no effect on the occurrence of F. graminearum compared with those of the untreated controls. Surprisingly, azoxystrobin and carbendazim significantly increased the total DON content in the harvested grain because they might have stimulated the amount of total DON production per Tri5 DNA. The fungicides Js399-19, tebuconazole, and the mixture of tebuconazole and thiram were the most effective in controlling FHB and reducing DON contamination of the wheat.