Cytochrome P450s from the Chinese white pine beetle, Dendroctonus armandi (Curculionidae: Scolytinae): Expression profiles of different stages and responses to host allelochemicals
Cytochrome P450s from the Chinese white pine beetle, Dendroctonus armandi (Curculionidae: Scolytinae): Expression profiles of different stages and responses to host allelochemicals
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DOI：
10.1016/j.ibmb.2015.08.004
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发表时间：
2015-10-01
影响因子：
3.8
通讯作者：
Chen, Hui
中科院分区：
文献类型：
--
作者：
Dai, Lulu;Ma, Mingyuan;Chen, Hui
树皮甲虫氧化寄主树上的防御性化感化学物质，将这些物质解毒并将其转化为信息素系统的组成部分。这种昆虫适应其化学环境的能力可能是通过细胞色素 P450（或 CYP）等主要解毒酶的作用来解释的。鉴定出 64 个编码 P450 的序列，并且发现大多数转录本在华山树蝽的幼虫、蛹和成虫中表达。为了获得有关这些基因如何帮助华曼迪克服宿主防御的信息，观察了两性之间和两性内部 CYP 基因转录水平的差异。在不同发育阶段、以华山松韧皮部为食和暴露于刺激物（(+/-)-α-蒎烯、(S)-(-)-(z-蒎烯、(S)-(-)-β-蒎烯、(+)-3-蒈烯、(+/-)-柠檬烯和松节油）8小时）中观察到显着差异。我们研究了性别和世代对华山松韧皮部的影响。 使用熏蒸剂暴露于暴露于与本构和诱导防御水平相当的浓度的宿主挥发物的个体的存活率，以了解甲虫耐受宿主防御化学物质的能力。这些树皮甲虫 CYP 基因的差异转录积累模式为了解华山甲虫与其宿主松树的生态相互作用提供了见解。 (C) 2015 Elsevier Ltd. 保留所有权利。
Bark beetles oxidize the defensive allelochemicals from their host trees to both detoxify and convert these materials into components of their pheromone system. The ability of this insect to adapt to its chemical environment might be explained by the action of major detoxification enzymes such as cytochrome P450s (or CYPs). Sixty-four sequences coding for P450s were identified, and most of the transcripts were found to be expressed in the larvae, pupae and adults of Dendroctonus armandi. To gain information on how these genes help D. armandi overcome the host defense, differential transcript levels of the CYP genes were observed between sexes and within the sexes. Significant differences were observed among developmental stages, in feeding on the phloem of Pinus armandi and in exposure to stimuli ((+/-)-alpha-pinene, (S)-(-)-(z-pinene, (S)-(-)-beta-pinene, (+)-3-carene, (+/-)-limonene and turpentine oil) for 8 h. We investigated the effect of sex and generations on the survivorship of individual D. armandi that were exposed to host volatiles at concentrations comparable to constitutive and induced levels of defense using fumigant exposure to understand the ability of the beetles to tolerate host defensive chemicals. The differential transcript accumulation patterns of CYP genes of these bark beetle provided insight into the ecological interactions of D. armandi with its host pine. (C) 2015 Elsevier Ltd. All rights reserved.