Regulation of the Arabidopsis anther transcriptome by DYT1 for pollen development
Regulation of the Arabidopsis anther transcriptome by DYT1 for pollen development
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DOI：
10.1111/j.1365-313x.2012.05104.x
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发表时间：
2012-11-01
期刊：
影响因子：
7.2
通讯作者：
Ma, Hong
中科院分区：
文献类型：
--
作者：
Feng, Baomin;Lu, Dihong;Ma, Hong
一些转录因子编码的基因已被证明是植物雄性育性所必需的，这表明转录调控是控制拟南芥花药发育的主要机制。功能异常绒毡层1（DYT 1）是一个bHLH转录因子，在调控绒毡层功能和花粉发育中起重要作用。在这里，我们比较野生型和dyt1突变体的年轻花药的转录组，表明DYT1是上游的至少22个基因编码的转录因子和调节大量的基因的表达，包括参与特定的代谢途径的基因。我们还表明，DYT1可以结合到DNA在体外的序列特异性的方式，并在体内诱导DYT1活性激活下游转录因子基因MYB35和MS1的表达。我们产生了DYT1SRDX转基因植物，其生育力显着降低，这意味着DYT1可能作为一个转录激活因子。此外，我们使用酵母双杂交试验表明，DYT1形成同源二聚体和异源二聚体与其他bHLH转录因子。我们的研究结果表明DYT1在调控花药转录组和功能，支持正常花粉发育中的重要作用。
Several genes encoding transcription factors have been shown to be essential for male fertility in plants, suggesting that transcriptional regulation is a major mechanism controlling anther development in Arabidopsis. DYSFUNCTIONAL TAPETUM 1 (DYT1), a putative bHLH transcription factor, plays a critical role in regulating tapetum function and pollen development. Here, we compare the transcriptomes of young anthers of wild-type and the dyt1 mutant, demonstrating that DYT1 is upstream of at least 22 genes encoding transcription factors and regulates the expression of a large number of genes, including genes involved in specific metabolic pathways. We also show that DYT1 can bind to DNA in a sequence-specific manner in vitro, and induction of DYT1 activity in vivo activated the expression of the downstream transcription factor genes MYB35 and MS1. We generated DYT1SRDX transgenic plants whose fertility was dramatically reduced, implying that DYT1 probably acts as a transcriptional activator. Furthermore, we used yeast two-hybrid assays to show that DYT1 forms homodimers and heterodimers with other bHLH transcription factors. Our results demonstrate the important role of DYT1 in regulating anther transcriptome and function, and supporting normal pollen development.