Maturation of collagen fibril network structure in tibial and femoral cartilage of rabbits
Maturation of collagen fibril network structure in tibial and femoral cartilage of rabbits
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DOI：
10.1016/j.joca.2009.11.007
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发表时间：
2010-03-01
影响因子：
7
通讯作者：
Helminen, H. J.
中科院分区：
文献类型：
--
作者：
Julkunen, P.;Iivarinen, J.;Helminen, H. J.
目的：关节软骨的结构和组成在发育和生长过程中以及响应于不同的负荷条件而变化。这些变化调节软骨的功能特性。我们研究了软骨胶原网络组织的成熟相关变化，作为组织深度的函数。设计：从六个年龄组收集雌性新西兰白色兔胫骨内侧平台和股骨内侧髁的关节软骨：4周（n = 30）、6周（n = 30）、3个月（n = 24）、6个月（n = 24）、9个月（n = 27）和18个月（n = 19）。胶原纤维的取向，平行性（各向异性）和光学延迟进行了分析与偏振光显微镜。在连续的年龄组和两个关节locations.Results：关节软骨的胶原纤维网络的深度方面的胶原组织的发展的差异进行了比较，在成熟过程中发生显着变化。如通过取向、平行性和延迟所评估的，在胫骨软骨中在4周龄和6周龄之间以及在股骨软骨中在6周龄和3个月之间注意到胶原结构的最显著变化，即，取向变得更接近表面，平行度和延迟增加，其中在深部区域中变化最显著。在6周龄时，胫骨软骨在中、深区比股骨软骨具有更接近表面的取向（P < 0.001），在整个组织深度上具有更高的平行度（P <0.001），而成熟后，股骨软骨在深区以上具有更平行于表面的取向（P < 0.01）。4周龄和6周龄时胫骨软骨胶原的光延迟值高于股骨软骨（P < 0.001），而在年龄较大时，股骨软骨的表浅区以下的阻滞比胫骨软骨的要高。在成熟过程中，关节软骨中的胶原组织有显著的调节，其在胫骨软骨中比在股骨软骨中发生得更早，并且在深层中最明显。(C)2009年国际骨关节炎研究学会。由爱思唯尔有限公司出版。保留所有权利。
Objective: The structure and composition of articular cartilage change during development and growth, as well as in response to varying loading conditions. These changes modulate the functional properties of cartilage. We studied maturation-related changes in the collagen network organization of cartilage as a function of tissue depth.Design: Articular cartilage from the tibial medial plateaus and femoral medial condyles of female New Zealand white rabbits was collected from six age-groups: 4 weeks (n = 30), 6 weeks (n = 30), 3 months (n = 24), 6 months (n = 24), 9 months (n = 27) and 18 months (n = 19). Collagen fibril orientation, parallelism (anisotropy) and optical retardation were analyzed with polarized light microscopy. Differences in the development of depth-wise collagen organization in consecutive age-groups and the two joint locations were compared statistically.Results: The collagen fibril network of articular cartilage undergoes significant changes during maturation. The most prominent changes in collagen architecture, as assessed by orientation, parallelism and retardation were noticed between the ages of 4 and 6 weeks in tibial cartilage and between 6 weeks and 3 months in femoral cartilage, i.e., orientation became more perpendicular-to-surface, and parallelism and retardation increased with changes being most prominent in the deep zone. At the age of 6 weeks, tibial cartilage had a more perpendicular-to-surface orientation in the middle and deep zones than femoral cartilage (P < 0.001) and higher parallelism throughout the tissue depth (P < 0.001), while femoral cartilage exhibited more parallel-to-surface orientation (P < 0.01) above the deep zone after maturation. Optical retardation of collagen was higher in tibial than in femoral cartilage at the ages of 4 and 6 weeks (P < 0.001), while at older ages, retardation below the superficial zone in the femoral cartilage became higher than in the tibial cartilage.Conclusions: During maturation, there is a significant modulation of collagen organization in articular cartilage which occurs earlier in tibial than in femoral cartilage, and is most pronounced in the deep zone. (C) 2009 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.