Growth inhibition and modulation of cell markers of melanoma by S-allyl cysteine.
Growth inhibition and modulation of cell markers of melanoma by S-allyl cysteine.
复制标题
S-烯丙基半胱氨酸对黑色素瘤细胞标志物的生长抑制和调节。
作者：
H. Takeyama;D. B. Hoon;R. Saxton;D. Morton;R. Irie
一种含硫的氨基酸化合物，S-烯丙基半胱氨酸（SAC），来自大蒜提取物抑制9人和小鼠黑色素瘤细胞系增殖的剂量依赖性的方式（1.2-10 mM）通过[3 H]胸苷掺入试验评估。三个对照人类淋巴母细胞样细胞系没有受到SAC浓度< 5 mM的抑制。在软琼脂试验中，四个人类黑色素瘤细胞系也显示出SAC对集落形成的剂量依赖性抑制。在这四种人黑素瘤和两种B16鼠黑素瘤亚系中，与相同的未处理细胞系相比，黑色素含量增加高达95%。SAC处理后细胞表面神经节苷脂、细胞分化和转化标志物的表达下降。还观察到显著的形态学变化，包括“扁平化和/或树突样伸长”。因此，SAC抑制细胞生长和增殖，并调节黑色素瘤的主要细胞分化标志物。
A sulfur-containing amino acid compound, S-allyl cysteine (SAC), derived from garlic extract inhibited proliferation of nine human and murine melanoma cell line in a dose-dependent manner (1.2-10 mM) assessed by a [3H]thymidine incorporation assay. Three control human lymphoblastoid cell lines were not inhibited by SAC concentrations < 5 mM. Four human melanoma cell lines in a soft-agar assay also showed dose-dependent inhibition of colony formation by SAC. Melanin content was increased up to 95% compared to the same untreated cell lines in these four human melanoma and two B16 murine melanoma sublines. Expression of cell surface gangliosides, cellular-differentiation and transformation markers, decreased after SAC treatment. Significant morphological changes including 'flattening and/or dendritic-like elongations' were also observed. Thus SAC inhibited cellular growth and proliferation and modulated major cell differentiation markers of melanoma.