Active and passive cation transport and L antigen hertogeneity in low potassium sheep red cells
Active and passive cation transport and L antigen hertogeneity in low potassium sheep red cells
复制标题
低钾绵羊红细胞的主动和被动阳离子转运及L抗原遗传性
DOI：
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发表时间：
1977
期刊：
影响因子：
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通讯作者：
C. Joiner
中科院分区：
文献类型：
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作者：
P. Lauf;BJ Steihl;C. Joiner
几条实验证据表明，低钾（LK）绵羊红细胞中的L抗原与单独的Na（+）K（+）泵通量相关，与抗L（l）对K（+）泄漏通量的作用不同，这意味着K（+）泄漏转运位点可能不会被L抗血清转化为活性泵。用胰蛋白酶处理 LK 红细胞完全消除了 K(+) 泵通量的刺激和抗 L 带来的哇巴因结合速率的增强。这种效果是由于与 LK 泵相关的 L(p) 决定簇被完全破坏所致，这从抗 L(p) 与胰蛋白酶处理的 LK 红细胞的结合完全失败中可以明显看出。通过预先与抗-L孵育，可以有效地保护L(p)抗原免受胰蛋白酶的攻击，这表明抗体结合和胰蛋白酶作用的位点可能在结构水平上紧密相邻。然而，胰蛋白酶处理不会干扰抗L(l)减少哇巴因不敏感的K(+)泄漏流入，也不会阻止抗L(ly)的结合，抗L(ly)是可能与抗L(l)相同的溶血活性L抗体。 L(p)和L(l)位点的功能独立性通过以下观察记录：抗L(l)仍然减少具有实验诱导的高钾浓度的LK细胞中的K(+)渗漏流入，在该浓度下K(+)泵通量被完全抑制，无论抗L(p)是否与与LK泵相关的L(p)抗原结合。
Several lines of experimental evidence are presented suggesting that the L antigens in low potassium (LK) sheep red cells are associated with separate Na(+)K(+) pump flux is distinct from the action of anti-L(l) on K(+) leak flux, implying that K(+) leak transport sites may not be converted into active pumps by the L antiserum. Treatment of LK red cells with trypsin completely abolished both the stimulation of K(+) pump flux and the enhancement of the rate of ouabain binding brought about by anti- L. That this effect is due to a total destruction of the L(p) determinant associated with the LK pump was evident from the complete failure of anti-L(p) to bind to trypsinized LK red cells. The L(p) antigen can be effectively protected against the trypsin attack by prior incubation with anti-L, indicating that the sites for antibody binding and trypsin action may be closely adjacent at the structural level. Trypsin treatment, however, did not interfere with anti-L(l) reducing ouabain insensitive K(+) leak influx, nor did it prevent binding of anti-L(ly), the hemolytically active L antibody which is probably identical with anti-L(l). The functional independence of the L(p) and L(l) sites was documented by the observation that anti-L(l) still reduced K(+) leak influx in LK cells with experimentally induced high potassium concentrations, at which K(+) pump flux is fully suppressed, whether or not anti-L(p) was binding to the L(p) antigen associated with the LK pump.