Zeptomole detection of streptavidin using carbon paste electrode and square-wave voltammetry
Zeptomole detection of streptavidin using carbon paste electrode and square-wave voltammetry
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DOI：
10.1002/elan.200603837
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发表时间：
2007-06-01
期刊：
影响因子：
3
通讯作者：
Kizek, Rene
中科院分区：
文献类型：
--
作者：
Petrlova, Jitka;Masarik, Michal;Kizek, Rene
鉴于方波伏安法分析的敏感性，我们比较了亲和素和链霉亲和素在亲和素-生物素技术中的适用性。在我们的初步实验中，我们发现在相同的实验条件和浓度下，链霉亲和素的电化学响应比亲和素高约100倍。因此，我们采用了两种方法来测定链霉亲和素--直接电化学池分析和吸附转移技术分析。在两种情况下，碳糊电极表面长达10分钟的累积都被确定为最佳。检测下限分别为0.3am(电化学池)和30am(ADTS)。
We compared the suitability of avidin and streptavidin for avidin-biotin technology in view of the sensitivity of the analysis using square-wave voltammetry. We found out during our preliminary experiments that streptavidin gave approximately 100 times higher electrochemical response in comparison with avidin at the same experimental conditions and concentration. Thus, we used two approaches for streptavidin determination - analysis directly in electrochemical cell and analysis by adsorptive transfer technique (AdTS). Ten minutes long accumulation on carbon paste electrode surface was ascertained as optimal in both cases. Limits of detection were 0.3 aM (electrochemical cell) and 30 aM (AdTS).