Faecal egg counts provide a reliable measure of Trichostrongylus tenuis intensities in free-living red grouse Lagopus lagopus scoticus
Faecal egg counts provide a reliable measure of Trichostrongylus tenuis intensities in free-living red grouse Lagopus lagopus scoticus
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DOI：
10.1079/joh2003220
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发表时间：
2004-03-01
影响因子：
1.6
通讯作者：
Hudson, PJ
中科院分区：
文献类型：
--
作者：
Seivwright, LJ;Redpath, SM;Hudson, PJ
在秋季，对红松鸡 (Lagopus lagopus Scoticus) 细毛圆线虫感染强度的不同鸡蛋计数方法的可靠性进行了调查，此时松鸡的寄生虫强度可能很高。还检查了使用这些方法的可能限制。研究发现，粪便卵计数可以准确估计 T. tenuis 蠕虫的强度，至少达到观察到的最大值 c。 8000只蠕虫。两种卵计数方法（涂片法和麦克马斯特法）给出了一致的结果，尽管与蠕虫强度的确切关系根据所使用的方法而有所不同。随着样品储存时间的增加，粪便虫卵计数显着减少，但虫卵计数可可靠地估计三周内的蠕虫强度。还发现盲肠中卵的浓度可以可靠地估计蠕虫强度。然而，冷冻肠道样本中的卵子计数不能用于估计蠕虫强度。这些结果得出的结论是，尽管存在一些局限性，粪便和盲肠虫卵计数仍为测量红松鸡中的细锥虫强度提供了有用且可靠的方法。
The reliability of different egg counting methods for estimating the intensity of Trichostrongylus tenuis infections in red grouse, Lagopus lagopus scoticus, was investigated in the autumn, when grouse may harbour high parasite intensities. Possible limitations to the use of these methods were also examined. Faecal egg counts were found to accurately estimate T. tenuis worm intensities, at least up to an observed maximum of c. 8000 worms. Two egg counting methods (smear and McMaster) gave consistent results, although the exact relationship with worm intensity differed according to the method used. Faecal egg counts significantly decreased with increasing length of sample storage time, but egg counts were reliable for estimating worm intensity for three weeks. The concentration of eggs in the caecum was also found to reliably estimate worm intensity. However, egg counts from frozen gut samples cannot be used to estimate worm intensities. These results conclude that, despite some limitations, faecal and caecum egg counts provide useful and reliable ways of measuring T. tenuis intensities in red grouse.