Circularly polarized light standards for investigations of collagen fiber orientation in bone.
Circularly polarized light standards for investigations of collagen fiber orientation in bone.
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DOI：
10.1002/ar.b.10031
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发表时间：
2003-09-01
影响因子：
--
通讯作者：
Riggs, Christopher M.
中科院分区：
文献类型：
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作者：
Bromage, Timothy G.;Goldman, Haviva M.;Riggs, Christopher M.
骨的正型双折射主要取决于骨胶原的取向。骨作为一种组织的生物力学功效在很大程度上取决于胶原纤维的优先取向和分布（以及矿物微晶的相应取向），并且越来越多的证据表明这种功效延伸到器官水平的功能。本研究有三个目的。首先是提供一个背景的圆偏振光（CPL）的研究骨胶原纤维取向的调查。本文综述了骨胶原纤维取向的意义、线性偏振光和CPL成像原理以及CPL在哺乳动物功能组织学研究中的历史。第二个是描述，在一些细节，有关的标本制备和成像的定量分析优先取向的胶原蛋白适当的方法考虑。这些包括切片透明度、切片厚度、照明系统的均匀性和CPL设备。最后，我们描述了一个灰度级标准的定量CPL有用，矿化火鸡腱，应根据要求提供给调查人员的基础上。当适当考虑标本制备和成像条件时，胶原纤维取向的定量评估可深入了解机械负荷对骨骼的影响。
Bone exhibits positive form birefringence dominated by and dependent upon the orientation of its collagen. The biomechanical efficacy of bone as a tissue is largely determined by collagen fibers of preferred orientation and distribution (and corresponding orientation of mineral crystallites), and evidence is accumulating to demonstrate that this efficacy extends to function at the organ level. This study has three aims. The first is to provide a background to the study of circularly polarized light (CPL) investigations of collagen fiber orientation in bone. The significance of preferred collagen fiber orientation in bone, linearly polarized light and CPL imaging principles, and a short history of CPL studies of mammalian functional histology are reviewed. The second is to describe, in some detail, methodological considerations relating to specimen preparation and imaging appropriate for the quantitative analysis of preferentially oriented collagen. These include section transparency, section thickness, the uniformity of the illuminating system, and CPL paraphernalia. Finally, we describe a grey-level standard useful for quantitative CPL, based upon mineralized turkey tendon, which shall be provided to investigators upon request. When due consideration is paid to specimen preparation and imaging conditions, quantitative assessment of collagen fiber orientation provides insight into the effects of mechanical loading on the skeleton.