Effect of long-term exposure to low-level toluene on airway inflammatory response in mice
Effect of long-term exposure to low-level toluene on airway inflammatory response in mice
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DOI：
10.1016/j.toxlet.2006.11.008
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发表时间：
2007-01-30
影响因子：
3.5
通讯作者：
Arashidani, Keiichi
中科院分区：
文献类型：
--
作者：
Fujimaki, Hidekazu;Yamamoto, Shoji;Arashidani, Keiichi
挥发性有机化合物是引起人体多种化学敏感性反应的主要物质。我们的实验室此前已表明，低浓度甲醛的暴露会导致小鼠产生免疫原性和神经源性炎症反应。本研究的目的是调查长期低水平甲苯暴露对小鼠肺部气道炎症反应的影响。我们将雌性 C3H 小鼠在全身暴露室中暴露于过滤空气 (0 ppm) 或 50 ppm 甲苯中，每天 6 小时，每周 5 天，持续 6 或 12 周。最后一次甲苯暴露后一天，我们收集每只小鼠的支气管肺泡灌洗液，并使用 ELISA 方法检查细胞浸润和细胞因子、趋化因子、神经营养因子和 P 物质的产生。我们发现暴露 6 周和 12 周的小鼠的总细胞和巨噬细胞数量均显着增加。此外，在暴露6周和12周的小鼠中，干扰素-γ和P物质的产生显着减少，神经生长因子未受影响。相比之下，仅暴露 12 周的小鼠的支气管肺泡灌洗液中神经营养素 3 的产生显着增加。我们的研究结果表明，小鼠长期（12 周）暴露于低浓度甲苯可通过神经信号传导调节气道炎症反应。 (c) 2006 Elsevier Ireland Ltd. 保留所有权利。
Volatile organic compounds are the main substances causing multiple chemical sensitivity reactions in human. Our laboratory has previously showed that the exposure of low-level formaldehyde causes immunogenic and neurogenic inflammatory responses in mice. The aim of the present study was to investigate the effect of long-term, low-level toluene exposure on airway inflammatory responses in mice lung. We exposed female C3H mice to filtered air (0 pprn) or 50 ppm of toluene for 6 h/day on 5 days/week for 6 or 12 weeks in the whole body exposure chamber. One day following the last toluene exposure, we collected bronchoalveolar lavage fluid from each mouse and examined cellular infiltration and production of cytokines, chemokines, neurotrophins and substance P by using ELISA method. We found that the number of total cells and macrophages increased significantly in both 6 and 12-week-exposed mice. In addition, the production of interferon-gamma and substance P were decreased significantly and nerve growth factor was not affected in both 6 and 12-week-exposed mice. In contrast, neurotrophin-3 production in bronchoalveolar lavage fluid was significantly increased only in 12-week-exposed mice. Our findings suggest that long-term (12-week) exposure of mice to low-level toluene modulates airway inflammatory response via neurological signaling. (c) 2006 Elsevier Ireland Ltd. All rights reserved.