Occurrence of chlorophyll allomers during virus-induced mortality and population decline in the ubiquitous picoeukaryote Ostreococcus tauri.
Occurrence of chlorophyll allomers during virus-induced mortality and population decline in the ubiquitous picoeukaryote Ostreococcus tauri.
复制标题
在普遍存在的超微核生物金牛骨球菌中，病毒引起的死亡和种群数量下降期间叶绿素异质体的出现。
DOI：
10.1111/1462-2920.13980
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发表时间：
2018
影响因子：
5.1
通讯作者：
Steele DJ
中科院分区：
文献类型：
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作者：
Steele DJ
在病毒感染和生长限制的picoeukaryoteOstreococcus tauri，我们研究了膜透性，氧化应激和叶绿素异构体（氧化产物）之间的关系。叶绿素异构体在O.牛磺酸与光系统II光化学的最大量子效率（Fv/Fm）、类胡萝卜素和活性氧以及膜渗透性平行，使用荧光探针（CM-H2 DCFDA和SYTOX-绿色）。病毒感染导致大量细胞溶解。48 h内牛磺酸细胞死亡。异源异构体羟基叶绿素的浓度在主要溶解事件后立即增加了16倍（相对于叶绿素a）。相比之下，由于生长限制导致的细胞死亡导致异源异构体产量相对于chl‐a增加两倍。两种异构体仅与O.牛磺内酯在病毒裂解后，并且不同于其他的异源异构体，在病毒裂解前或由于生长限制的细胞死亡期间未观察到。相反，在指数增长的O中，相对于chl‐a，发现组分chl‐ aP 276的浓度最高。金牛座。所描述的组件有可能作为浮游植物死亡率和人口增长模式的指标。
During viral infection and growth limitation of the picoeukaryoteOstreococcus tauri, we examined the relationship between membrane permeability, oxidative stress and chlorophyll allomers (oxidation products). Chlorophyll allomers were measured in batch‐cultures ofO. tauriin parallel with maximum quantum efficiency of photosystem II photochemistry (Fv/Fm), carotenoids, and reactive oxygen species and membrane permeability using fluorescent probes (CM‐H2DCFDA and SYTOX‐Green). Viral infection led to mass cell lysis of theO. tauricells within 48 h. The concentration of the allomer hydroxychlorophyllapeaked with a 16‐fold increase (relative to chlorophyll‐a) just after the major lysis event. In contrast, cell death due to growth limitation resulted in a twofold increase in allomer production, relative to chl‐a. Two allomers were detected solely in association withO. tauridebris after viral lysis, and unlike other allomers were not observed before viral lysis, or during cell death due to growth limitation. Conversely, the component chl‐aP276was found in the highest concentrations relative to chl‐a, in exponentially growingO. tauri. The components described have potential as indicators of mode of phytoplankton mortality, and of population growth.