DNA condensation by protamine and arginine-rich peptides: Analysis of toroid stability using single DNA molecules
DNA condensation by protamine and arginine-rich peptides: Analysis of toroid stability using single DNA molecules
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DOI：
10.1002/(sici)1098-2795(200006)56:2
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发表时间：
2000-06-01
影响因子：
2.5
通讯作者：
Corzett, M
中科院分区：
文献类型：
--
作者：
Balhorn, R;Brewer, L;Corzett, M
体细胞和精子都能吸收外源DNA，但其整合频率通常较低。精子染色质和合成的DNA-鱼精蛋白复合物的扫描探针显微镜研究表明，螺旋的DNA成环形亚基，在成熟的精子细胞中开始准备其基因组交付到鸡蛋的过程，可以模仿简单地添加鱼精蛋白的DNA在体外。DNA-鱼精蛋白复合物对核酸酶消化的抵抗力增加以及它们与天然精子染色质的结构相似性表明，鱼精蛋白包装DNA可能为提高精子摄取DNA的效率提供新的途径。用单独的DNA分子进行的解凝聚实验提供了使用鲑鱼精蛋白和较小的富含精氨酸的肽产生的环状体的稳定性的直接测量。这些实验表明，精蛋白相关序列的精氨酸含量可以对其从DNA解离的速率产生显著影响。这项技术及其提供的信息可用于鉴定可与DNA结合的鱼精蛋白类似物，以提高精子和其他细胞对其的吸收效率。出版2000 Wiley-Liss，Inc.（匕首）
Both somatic cells and sperm have been shown to take up exogenous DNA, but the frequency of its integration is usually low. Scanning probe microscopy studies of sperm chromatin and synthetic DNA-protamine complexes indicate that the coiling of DNA into toroidal subunits, a process initiated in the maturing spermatid to prepare its genome for delivery into the egg, can be mimicked by simply adding protamine to DNA in vitro. The increased resistance of DNA-protamine complexes to nuclease digestion and their structural similarity to native sperm chromatin suggest that the packaging of DNA by protamine might offer a new approach for improving the efficiency of DNA uptake by sperm. Decondensation experiments performed with individual DNA molecules have provided a direct measure of the stability of toroids produced using salmon protamine and smaller arginine-rich peptides. These experiments show that the arginine content of protamine-related sequences can have a dramatic effect on their rate of dissociation from DNA. This technique and the information it provides can be used to identify protamine analogs that can be bound to DNA to increase the efficiency of its uptake by sperm and other cells. Published 2000 Wiley-Liss, Inc.(dagger)