Heavy-meromyosin-decoration of microfilaments from Mougeotia protoplasts
Heavy-meromyosin-decoration of microfilaments from Mougeotia protoplasts
复制标题
Mougeotia原生质体微丝的重meromyosin修饰
作者：
Karin Klein;G. Wagner;M. Blatt
绿色转板藻（Mougeotia）的细胞壁用粗酶和纤维素酶进行酶解。将释放的原生质体涂布在聚-L-鸟氨酸、formvar-碳包被的网格上，并收集细胞片段用于结构表征。可见大量5-7 nm的细丝，其可能被重肌球蛋白（HMM）装饰，所述重肌球蛋白是特异性结合肌动蛋白的肌肉肌球蛋白的消化产物，支持以下假设：这些藻类中光敏色素介导的叶绿体运动由肌动球蛋白的收缩复合物驱动。
The cell wall of the green algaMougeotiawas enzymatically digested by macerase and cellulysin. Released protoplasts were spread on poly-L-ornithine, formvar-carbon-coated grids, and cell fragments were collected for structural characterization. Large numbers of 5–7 nm filaments are seen which may be decorated with heavy meromyosin (HMM), a digest product of muscle myosin that binds specifically to actin, supporting the hypothesis that the phytochrome-mediated chloroplast movements in these algae are driven by a contractile complex of actomyosin.