Establishment of Cell Lines Derived From the Genus Macaca Through Controlled Expression of Cell Cycle Regulators
Establishment of Cell Lines Derived From the Genus Macaca Through Controlled Expression of Cell Cycle Regulators
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DOI：
10.1002/jcb.24963
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发表时间：
2015-02-01
影响因子：
4
通讯作者：
Fukuda, Tomokazu
中科院分区：
文献类型：
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作者：
Kuroda, Kengo;Kiyono, Tohru;Fukuda, Tomokazu
非人灵长类动物是研究人类疾病的有用动物模型。然而，与从其他实验动物建立的细胞系的数量相比，从非人灵长类动物建立的细胞系的数量非常有限。非人类灵长类动物细胞系的建立将允许在将实验转移到灵长类动物之前对这些细胞系进行药物测试。在这项研究中，我们建立了非人灵长类动物的原代细胞系，通过引入CDK 4 R24 C，细胞周期蛋白D1，和hTERT的基因。这些细胞系比原代细胞增殖更快，并绕过细胞衰老。核型分析表明，永生化细胞系的染色体模式是完整的。此外，我们表明，引入的基因的表达可以通过Tet-Off系统与强力霉素的添加进行精确控制。目前的研究表明，引入CDK 4 R24 C，细胞周期蛋白D1，和hTERT基因是建立非人灵长类动物细胞系的有效方法。(C)2014 Wiley Periodicals，Inc.
Nonhuman primates are useful animal models for the study of human diseases. However, the number of established cell lines from nonhuman primates is quite limited compared with the number established from other experimental animals. The establishment of nonhuman primate cell lines would allow drug testing on those cell lines before moving experiments into primates. In this study, we established nonhuman primate primary cell lines by introducing the genes for CDK4R24C, cyclin D1, and hTERT. These cell lines proliferated more rapidly than primary cells and bypassed cellular senescence. Karyotype analysis showed that the chromosome patterns were intact in the immortalized cell lines. Furthermore, we showed that the expression of introduced genes could be precisely controlled through the Tet-Off system with the addition of doxycycline. The present study shows that introduction of the CDK4R24C, cyclin D1, and hTERT genes are effective methods of establishing nonhuman primate cell lines. (C) 2014 Wiley Periodicals, Inc.