Ribosomal frameshifting used in influenza A virus expression occurs within the sequence UCC_UUU_CGU and is in the +1 direction.
Ribosomal frameshifting used in influenza A virus expression occurs within the sequence UCC_UUU_CGU and is in the +1 direction.
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流感A病毒表达中使用的核糖体框架发生在序列UCC_UUU_CGU内，并且位于+1方向。
DOI：
10.1098/rsob.120109
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发表时间：
2012-10
期刊：
影响因子：
5.8
通讯作者：
Atkins JF
中科院分区：
文献类型：
--
作者：
Firth AE;Jagger BW;Wise HM;Nelson CC;Parsawar K;Wills NM;Napthine S;Taubenberger JK;Digard P;Atkins JF
程序性核糖体移码被用于许多病毒基因和一些细胞基因的表达。在真核系统中，最充分表征的机制涉及X_XXY_YYZ基序上的-1串联tRNA滑动。相比之下，程序性+1（或-2）滑动的机制更多样化，而且往往特征不佳。最近，在甲型流感病毒中发现了一个新的基因PA-X，并发现其通过向+1阅读框的移位来表达。在这里，我们确定，通过质谱分析，无论是网站（UCC_UUU_CGU）和方向（+1）的移码，参与PA-X的表达。在之前提出通过+1移码表达的其他病毒基因中发现了相关位点。当这些病毒感染昆虫（慢性蜜蜂麻痹病毒）、植物（斐济病毒和汞齐病毒）和脊椎动物（甲型流感病毒）时，这些基序可能形成一类新的+1移码诱导序列，这些序列在多种真核生物中具有活性。
Programmed ribosomal frameshifting is used in the expression of many virus genes and some cellular genes. In eukaryotic systems, the most well-characterized mechanism involves –1 tandem tRNA slippage on an X_XXY_YYZ motif. By contrast, the mechanisms involved in programmed +1 (or −2) slippage are more varied and often poorly characterized. Recently, a novel gene, PA-X, was discovered in influenza A virus and found to be expressed via a shift to the +1 reading frame. Here, we identify, by mass spectrometric analysis, both the site (UCC_UUU_CGU) and direction (+1) of the frameshifting that is involved in PA-X expression. Related sites are identified in other virus genes that have previously been proposed to be expressed via +1 frameshifting. As these viruses infect insects (chronic bee paralysis virus), plants (fijiviruses and amalgamaviruses) and vertebrates (influenza A virus), such motifs may form a new class of +1 frameshift-inducing sequences that are active in diverse eukaryotes.
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影响因子：
5.4
作者：
Amorim, Maria Joao;Bruce, Emily A.;Digard, Paul
通讯作者：
Digard, Paul
影响因子：
5.8
作者：
Bekaert, Michael;Atkins, John F.;Baranov, Pavel V.
通讯作者：
Baranov, Pavel V.
影响因子：
64.8
作者：
CRAIGEN, WJ;CASKEY, CT
通讯作者：
CASKEY, CT
DOI：
10.1099/vir.0.042499-0
发表时间：
2012-07
期刊：
The Journal of general virology
影响因子：
--
作者：
Firth AE;Brierley I
通讯作者：
Brierley I
DOI：
10.1073/pnas.1106999108
发表时间：
2011-10-11
影响因子：
11.1
作者：
Chen, Chunlai;Stevens, Benjamin;Goldman, Yale E.
通讯作者：
Goldman, Yale E.