Attenuation of and protection induced by a leucine auxotroph of Mycobacterium tuberculosis
Attenuation of and protection induced by a leucine auxotroph of Mycobacterium tuberculosis
复制标题
DOI：
10.1128/iai.68.5.2888-2898.2000
复制
发表时间：
2000-05-01
影响因子：
3.1
通讯作者：
Bloom, BR
中科院分区：
文献类型：
--
作者：
Hondalus, MK;Bardarov, S;Bloom, BR
结核分枝杆菌的减毒突变体代表了预防结核病的潜在疫苗候选物。已知多种细菌的营养缺陷型在体内被减毒，但仍提供针对野生型生物体攻击的保护。M.通过等位基因交换，用缺失了359 bp的基因的突变拷贝替换编码苹果酸异丙酯异构酶的野生型leuD（Rv2987c），产生了需要外源亮氨酸补充以在体外生长的菌株。回复原养型的频率为
Attenuated mutants of Mycobacterium tuberculosis represent potential vaccine candidates for the prevention of tuberculosis, It is known that auxotrophs of a variety of bacteria are attenuated in vivo and yet pro,ide protection against challenge with wild-type organisms. A leucine auxotroph of M. tuberculosis was created by allelic exchange, replacing wild-type leuD (Rv2987c), encoding isopropyl malate isomerase, with a mutant copy of the gene in which 359 bp had been deleted, creating a strain requiring exogenous leucine supplementation for growth in vitro. The frequency of reversion to prototrophy was