Recent thymic emigrants and subsets of naive and memory T cells in the circulation of patients with head and neck cancer
Recent thymic emigrants and subsets of naive and memory T cells in the circulation of patients with head and neck cancer
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DOI：
10.1016/j.clim.2004.12.011
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发表时间：
2005-07-01
影响因子：
8.6
通讯作者：
Whiteside, TL
中科院分区：
文献类型：
--
作者：
Kuss, I;Schaefer, C;Whiteside, TL
肿瘤患者外周血中CD8(+)T淋巴细胞的凋亡是一种常见现象。通过胸腺输出的T细胞或由抗原驱动的循环T细胞扩增可以补偿细胞凋亡，从而使其稳态正常化。本文对39例头颈癌(HNC)患者和33例年龄匹配的对照组(NC)外周血中T细胞受体切除环(TREC)鉴定的新近胸腺移民者(RTE)和幼稚和记忆性T细胞亚群的频率进行了研究。用实时荧光定量聚合酶链式反应检测TREC数量，用流式细胞仪检测CD8(+)、CD45RO(-)、CD27(+)或CD4(+)CD45RO(-)、CD27(+)T细胞亚群。癌症患者TREC数量和幼稚CD8(+)和CD4(+)T细胞亚群比例的年龄相关性下降显著高于正常对照组。相比之下，与正常对照组相比，癌症患者的记忆隔间扩大了，CD4(+)CD45RO(+)T细胞比例增加，但CD8(+)CD45RO(+)T细胞比例没有增加。在非ED患者中，这些改变并未正常化。这些数据表明，胸腺输出量减少和初始CD8(+)T细胞的快速更新是HNC患者淋巴细胞稳态改变的原因。这种缺陷在根治治疗后会持续很长时间，可能会导致癌症患者的免疫细胞失调。(C)2005 Elsevier Inc.保留所有权利。
Apoptosis of circulating CD8(+) T lymphocytes is a frequent finding in patients with cancer. T-cell output by the thymus or antigen-driven expansion of circulating T cells could compensate for apoptosis and thus normalize their homeostasis. We studied the frequency of recent thymic emigrants (RTE) identified by T-cell receptor excision circles (TREC) and of naive and memory T-cell subsets in peripheral blood samples obtained from 39 patients with head and neck cancer (HNC) and 33 age-matched controls (NC). TREC numbers were determined by real-time quantitative PCR, and CD8(+)CD45RO(-)CD27(+) or CD4(+)CD45RO(-)CD27(+) T-cell subsets were quantified by flow cytometry. Age-associated decreases in TREC numbers and proportions of naive CD8(+) and CD4(+) T-cell subsets were significantly greater in cancer patients than NC. In contrast, the memory compartment was expanded, with increased proportions of CD4(+)CD45RO(+) but not CD8(+)CD45RO(+) T cells, in cancer patients vs. NC. These alterations did not normalize in patients who were NED. The data suggest that lower thymic output combined with rapid turnover of naive CD8(+) T cells account for altered lymphocyte homeostasis in HNC patients. The defect persists long after curative treatments and may contribute to immune cell dysregulation in patients with cancer. (c) 2005 Elsevier Inc. All rights reserved.