Alexa Fluor 488 as an iron sensing molecule and its application in PEBBLE nanosensors
Alexa Fluor 488 as an iron sensing molecule and its application in PEBBLE nanosensors
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DOI：
10.1039/b414189j
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发表时间：
2005-01-01
期刊：
影响因子：
4.2
通讯作者：
Kopelman, R
中科院分区：
文献类型：
--
作者：
Sumner, JP;Kopelman, R
Molecular Probes的Alexa Fluor染料因其理想的荧光特性而通常用于生物标记，但在这里我们详细介绍了Alexa Fluor 488对游离铁的纳摩尔灵敏度。此外，通过微乳液方法将染料包封到聚合物纳米球中，产生< 100 nm的颗粒。这些纳米传感器，PEBBLEs（由生物定位嵌入封装的探针）具有微摩尔灵敏度，并且对其他生物感兴趣的金属离子不敏感。
Molecular Probes' Alexa Fluor dyes are generally used for biological labeling because of their ideal fluorescent properties, but here we detail Alexa Fluor 488's nanomolar sensitivity to free iron. Furthermore, the dye has been encapsulated into a polymer nanosphere by a microemulsion method, producing < 100 nm particles. These nanosensors, PEBBLEs (Probe Encapsulated By Biologically Localized Embedding) have micromolar sensitivity and are non-responsive to other metal ions of biological interest.