Regulation of both the structure and function by a de novo designed disulfide bond: a case study of heme proteins in myoglobin
Regulation of both the structure and function by a de novo designed disulfide bond: a case study of heme proteins in myoglobin
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从头设计的二硫键对结构和功能的调节：肌红蛋白中血红素蛋白的案例研究
DOI：
10.1039/c8cc01646a
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发表时间：
2018
影响因子：
4.9
通讯作者：
Lin Ying Wu
中科院分区：
文献类型：
--
作者：
Yin Lu Lu;Yuan Hong;Du Ke Jie;He Bo;Gao Shu Qin;Wen Ge Bo;Tan Xiangshi;Lin Ying Wu
肌红蛋白中从头设计的分子内二硫键，类似于细胞红蛋白中的分子内二硫键，但没有结构证据，首次通过X射线结构得到证实，并被证明可以调节该蛋白的结构和功能，满足人工脱卤过氧化物酶的设计，其活性超过天然酶。
A de novo designed intramolecular disulfide bond in myoglobin, resembling that in cytoglobin without structural evidence, was confirmed by an X-ray structure for the first time and was demonstrated to regulate both the structure and function of this protein, which fulfills the design of an artificial dehaloperoxidase, with an activity exceeding that of a native enzyme.