Intestinal infection due to enteroaggregative Escherichia coli among human immunodeficiency virus-infected persons
Intestinal infection due to enteroaggregative Escherichia coli among human immunodeficiency virus-infected persons
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DOI：
10.1086/315885
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发表时间：
2000-11-01
影响因子：
6.4
通讯作者：
Weber, R
中科院分区：
文献类型：
--
作者：
Durrer, P;Zbinden, R;Weber, R
为探讨肠聚集性大肠埃希菌(EAggEC)在人类免疫缺陷病毒(HIV)感染者中的致病作用，对68例无腹泻和III例腹泻的门诊患者进行了评估。粪便标本的检测包括HeLa细胞黏附试验和EAggEC聚合酶链式反应(PCR)检测，通过多因素分析，pCVD432基因、黏附表型和患者特征与腹泻的发生相关。EAggEC聚合酶链式反应和黏附试验阳性者分别为7例(6%)和24例(22%)，无症状对照组分别为1例(1%)和21例(31%)。EAggEC阳性菌株的临床表现是非特异性的；EAggEC感染与CD4淋巴细胞计数无关。在pCVD432基因分型中，5株(71%)对复方新诺明和氨苄西林耐药，1株对环丙沙星耐药。总体而言，pCVD432PCR阳性的大肠埃希菌是与腹泻相关的最常见的肠道微生物，黏附试验结果与腹泻无关。
To investigate the pathogenic role of enteroaggregative Escherichia coli (EAggEC) among human immunodeficiency virus-infected persons, iii outpatients with and 68 without diarrhea were evaluated. Examination of stool samples included the HeLa cell adherence assay and an EAggEC polymerase chain reaction (PCR) assay using primers complementary for the plasmid locus CVD432, The pCVD432 genotype, adherence phenotype, and patient characteristics were correlated with occurrence of diarrhea by multivariate analyses. EAggEC PCR and adherence assays were positive in 7 (6%) and 24 (22%) patients with diarrhea and in 1 (1%) and 21 (31%) asymptomatic control patients, respectively. Clinical manifestations associated with EAggEC PCR-positive isolates were nonspecific; EAggEC infections were independent of CD4 lymphocyte counts. Of the pCVD432 genotype, 5 (71%) of 7 were resistant to cotrimoxazole and ampicillin, and 1 strain was resistant to ciprofloxacin. Overall, pCVD432 PCR-positive E. coli was the most prevalent intestinal organism associated with diarrhea, The adherence assay results did not correlate with diarrhea.