Composition and abundance of microbiota in the pharynx in patients with laryngeal carcinoma and vocal cord polyps
Composition and abundance of microbiota in the pharynx in patients with laryngeal carcinoma and vocal cord polyps
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喉癌和声带息肉患者咽部微生物群的组成和丰度
DOI：
10.1007/s12275-017-6636-8
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发表时间：
2017-08-01
影响因子：
3
通讯作者：
Zhou, Liang
中科院分区：
文献类型：
--
作者：
Gong, Hongli;Wang, Boyan;Zhou, Liang
咽部是微生物定植的重要部位，但该部位的细菌群体相对未通过非培养方法进行探索。本研究的目的是表征咽部的微生物群结构。使用来自68名喉癌受试者和28名声带息肉受试者的拭子样本，使用16S rRNA基因文库的焦磷酸测序来表征咽部微生物群。总体而言，主要门是厚壁菌门，链球菌是咽部群落的优势属。其中链球菌属、梭杆菌属、普雷沃菌属、颗粒菌属和韦荣球菌属共9个核心操作分类单位占总序列的21.3%。然而，喉癌和声带息肉患者咽部的细菌群落没有差异。厚壁菌的相对丰度与梭菌、变形菌、放线菌和拟杆菌呈负相关。相关性在属的水平上是明显的，链球菌的相对丰度与梭杆菌属、细毛菌属、奈瑟菌属、放线菌属和普雷沃菌属呈负相关。本研究提供了咽拭子样本中微生物群的总体结构概况。链球菌和其他细菌群落之间存在负相关性，表明咽部微生物群之间可能存在潜在的拮抗作用。
The pharynx is an important site of microbiota colonization, but the bacterial populations at this site have been relatively unexplored by culture-independent approaches. The aim of this study was to characterize the microbiota structure of the pharynx. Pyrosequencing of 16S rRNA gene libraries was used to characterize the pharyngeal microbiota using swab samples from 68 subjects with laryngeal cancer and 28 subjects with vocal cord polyps. Overall, the major phylum wasFirmicutes, withStreptococcusas the predominant genus in the pharyngeal communities. Nine core operational taxonomic units detected fromStreptococcus,Fusobacterium,Prevotella,Granulicatella, andVeillonellaaccounted for 21.3% of the total sequences detected. However, there was no difference in bacterial communities in the pharynx from patients with laryngeal cancer and vocal cord polyps. The relative abundance ofFirmicuteswas inversely correlated withFusobacteria,Proteobacteria,Actinobacteria, andBacteroidetes. The correlation was evident at the genus level, and the relative abundance ofStreptococcuswas inversely associated withFusobacterium,Leptotrichia,Neisseria,Actinomyces, andPrevotella. This study presented a profile for the overall structure of the microbiota in pharyngeal swab samples. Inverse correlations were found between Streptococcus and other bacterial communities, suggesting that potential antagonism may exist among pharyngeal microbiota.