De Novo Transcriptome Sequencing of the Deep-Sea-Derived Fungus Dichotomomyces cejpii and Analysis of Gliotoxin Biosynthesis Genes.
De Novo Transcriptome Sequencing of the Deep-Sea-Derived Fungus Dichotomomyces cejpii and Analysis of Gliotoxin Biosynthesis Genes.
复制标题
深海真菌 Dichotomomyces cejpii 的从头转录组测序和胶霉毒素生物合成基因分析。
DOI：
10.3390/ijms19071910
复制
发表时间：
2018-06-29
影响因子：
5.6
通讯作者：
Li S
中科院分区：
文献类型：
--
作者：
Ye W;Zhang W;Liu T;Huang Z;Zhu M;Chen Y;Li H;Li S
胶霉毒素是由真菌产生的一种表聚硫代二氧代哌嗪（epipolythiodioxopiperazine，ETP）毒素，具有抗肝纤维化、抗肿瘤、抗真菌、抗病毒、抗氧化、免疫调节等生物活性。最近，细胞毒性胶毒素分离自深海来源的真菌，Dichotomomyces cejpii。然而，在D. cejpii仍不清楚。本研究对D.使用Illumina Hiseq 2000对cejpii进行测序。D.从9.73GB的干净读段获得CEJPII。注释了10个与胶胶毒素生物合成相关的基因。实时定量聚合酶链反应（qRT-PCR）检测胶质毒素相关基因的表达水平。GliG基因，编码谷胱甘肽S-转移酶（DC-GST）; GliI，编码氨基转移酶（DC-AI）;和GliO，编码醛还原酶（DC-AR），克隆和表达，纯化，并进行了表征。结果提示DC-GST、DC-AT和DC-AR在胶质毒素的生物合成中起重要作用。本研究对胶粘毒素生物合成相关基因的研究，为胶粘毒素的广泛应用奠定了分子基础。在未来的生物医药产业中。
Gliotoxin, produced by fungi, is an epipolythiodioxopiperazine (ETP) toxin with bioactivities such as anti-liver fibrosis, antitumor, antifungus, antivirus, antioxidation, and immunoregulation. Recently, cytotoxic gliotoxins were isolated from a deep-sea-derived fungus, Dichotomomyces cejpii. However, the biosynthetic pathway for gliotoxins in D. cejpii remains unclear. In this study, the transcriptome of D. cejpii was sequenced using an Illumina Hiseq 2000. A total of 19,125 unigenes for D. cejpii were obtained from 9.73 GB of clean reads. Ten genes related to gliotoxin biosynthesis were annotated. The expression levels of gliotoxin-related genes were detected through quantitative real-time polymerase chain reaction (qRT-PCR). The GliG gene, encoding a glutathione S-transferase (DC-GST); GliI, encoding an aminotransferase (DC-AI); and GliO, encoding an aldehyde reductase (DC-AR), were cloned and expressed, purified, and characterized. The results suggested the important roles of DC-GST, DC-AT, and DC-AR in the biosynthesis of gliotoxins. Our study on the genes related to gliotoxin biosynthesis establishes a molecular foundation for the wider application of gliotoxins from D. cejpii in the biomedical industry in the future.
登录
查看更多内容
影响因子：
5.4
作者：
Nguyen VT;Lee JS;Qian ZJ;Li YX;Kim KN;Heo SJ;Jeon YJ;Park WS;Choi IW;Je JY;Jung WK
通讯作者：
Jung WK
西兰花（Brassica oleracea var. italic）种子和芽中转录组和芥子油苷代谢的 RNA 测序分析
影响因子：
3.7
作者：
Gao J;Yu X;Ma F;Li J
通讯作者：
Li J
影响因子：
6.7
作者：
Schrettl M;Carberry S;Kavanagh K;Haas H;Jones GW;O'Brien J;Nolan A;Stephens J;Fenelon O;Doyle S
通讯作者：
Doyle S
DOI：
10.1016/j.bbrc.2007.05.139
发表时间：
2007-08-03
影响因子：
3.1
作者：
Choi, Hee Shim;Shim, Joong Sup;Kwon, Ho Jeong
通讯作者：
Kwon, Ho Jeong
影响因子：
15
作者：
Forseth, Ry R.;Fox, Ellen M.;Chung, DaWoon;Howlett, Barbara J.;Keller, Nancy P.;Schroeder, Frank C.
通讯作者：
Schroeder, Frank C.