SEQUENCING OF PEPTIDES IN A TIME-OF-FLIGHT MASS-SPECTROMETER - EVALUATION OF POSTSOURCE DECAY FOLLOWING MATRIX-ASSISTED LASER-DESORPTION IONIZATION (MALDI)
SEQUENCING OF PEPTIDES IN A TIME-OF-FLIGHT MASS-SPECTROMETER - EVALUATION OF POSTSOURCE DECAY FOLLOWING MATRIX-ASSISTED LASER-DESORPTION IONIZATION (MALDI)
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DOI：
10.1016/0168-1176(93)03876-n
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发表时间：
1994-02-24
期刊：
影响因子：
--
通讯作者：
SPENGLER, B
中科院分区：
文献类型：
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作者：
KAUFMANN, R;KIRSCH, D;SPENGLER, B
在基质辅助激光解吸电离（MALDI）中，大部分分析物离子在场自由漂移路径中发生后源衰变（PSD）。利用改进的两级反射器，记录了中等大小的线性肽（高达2800 u）的子离子飞行时间光谱，其中包含完整的序列信息。碎片离子的精密度、准确度和质量分辨率几乎与在四扇形仪器中进行的高能CAD研究一样好。仪器灵敏度至少提高了一个数量级。在反射飞行时间质谱（RETOF-MS）中，PSD产物的解理模式与高能和低能CAD所得到的不同。在本仪器中，通过改变仪器参数，可以很容易地在同一实验中比较地研究与高能和低能CAD在能量上相当的条件。发现PSD的激活机制在很大程度上取决于早期羽流膨胀时加速场引起的碰撞事件（离子/中性）。讨论了MALDI后PSD分析的发展前景。
In matrix assisted laser desorption ionisation (MALDI) a large fraction of analyte ions undergo postsource decay (PSD) during flight in the field free drift path. By means of a modified two-stage reflectron, daughter ion time-of-flight spectra of medium sized linear peptides (up to 2800 u) were recorded containing full sequence information. Precision, accuracy and mass resolution of fragment ions were almost as good as obtained in high energy CAD studies performed in four-sector instruments. Instrumental sensitivity was better by at least one order of magnitude. In reflectron time-of-flight mass spectrometry (RETOF-MS) the cleavage pattern of PSD products is different from that obtained by high energy and low energy CAD. In our instrument, conditions which were energetically comparable to high energy and low energy CAD could easily and comparatively be studied in the same experiment by varying instrumental parameters. Activation mechanisms of PSD were found to be largely determined by collisional events (ion/neutral) induced by the acceleration field during early plume expansion. Future potentials of PSD analysis after MALDI are discussed.