Agrobacterium rhizogenes-mediated transformation of Taraxacum platycarpum and changes of morphological characters
Agrobacterium rhizogenes-mediated transformation of Taraxacum platycarpum and changes of morphological characters
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DOI：
10.1007/s00299-004-0763-5
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发表时间：
2004-06-01
影响因子：
6.2
通讯作者：
Choi, YE
中科院分区：
文献类型：
--
作者：
Lee, MH;Yoon, ES;Choi, YE
通过用发根农杆菌 15834 感染，从蒲公英幼苗中有效诱导转化的毛状根。与茎 (32.7+/-4.8%) 或子叶 (16.2+/-5.7%) 相比，根外植体以更高的频率 (76.5+/-3.5%) 产生转化根。在不含生长调节剂的培养基上，毛根表现出主动伸长和高分枝。比较未转化的不定根和转化的毛状根的植物再生能力。在无激素培养基上，转化根形成不定芽的频率（88.5+/-9.8%）远高于非转化根（31.7+/-9.5%）。毛状根衍生的不定芽在不含生长调节剂的培养基上容易生根，但在非转化芽上未观察到生根。通过PCR和Southern杂交证实rol基因已稳定导入蒲公英植物中。与相应的野生型（非转基因）植物相比，转基因植株在形态上表现出相当大的差异。由转化根形成的幼苗具有大量须根和丰富的侧枝，而不是非转化植物中加厚的主根。观察到的差异可能反映了引入 rol 基因对根形态特征的改变。
Transformed hairy roots were efficiently induced from seedlings of Taraxacum platycarpum by infection with Agrobacterium rhizogenes 15834. Root explants produced transformed roots at a higher frequency (76.5+/-3.5%) as compared to stem (32.7+/-4.8%) or cotyledon (16.2+/-5.7%). Hairy roots exhibited active elongation with high branching of roots on growth regulator-free medium. The competence of plant regeneration from non-transformed adventitious roots and transformed hairy roots was compared. The frequency of adventitious shoot formation from transformed roots was much higher (88.5+/-9.8%) than that of non-transformed roots (31.7 +/-9.5%) on hormone-free medium. Rooting of hairy root-derived adventitious shoots occurred easily on growth regulator-free medium but no rooting was observed on non-transformed shoots. The stable introduction of rol genes into Taraxacum plants was confirmed by PCR and Southern hybridization. Transgenic plantlets showed considerable differences in their morphology when compared to the corresponding wild-type (non-transgenic) plants. Plantlets formed from transformed roots had numerous fibrous roots with abundant lateral branches instead of the thickened taproots in non-transformed plants. The differences observed may reflect the modification of morphological root characters by introduction of rol genes.