A Novel Isoform of Syntaxin-binding Protein Homologous to Yeast Sec1 Expressed Ubiquitously in Mammalian Cells (*)
A Novel Isoform of Syntaxin-binding Protein Homologous to Yeast Sec1 Expressed Ubiquitously in Mammalian Cells (*)
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一种与酵母 Sec1 同源的突触融合蛋白结合蛋白的新亚型，在哺乳动物细胞中普遍表达 (*)
DOI：
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发表时间：
1995
影响因子：
4.8
通讯作者：
Y. Oka
中科院分区：
文献类型：
--
作者：
H. Katagiri;J. Terasaki;T. Murata;H. Ishihara;T. Ogihara;K. Inukai;Y. Fukushima;M. Anai;M. Kikuchi;J. Miyazaki;Y. Yazaki;Y. Oka
munc-18/n-Sec1/rbSec1 是酵母 Sec1p 蛋白的大脑同源物，被认为参与调节突触小泡的对接和融合。我们筛选了源自胰腺 β 细胞的 MIN6 细胞系的小鼠 cDNA 文库，寻找其新亚型，并鉴定了编码 593 个氨基酸蛋白质的 cDNA，与 munc-18/n-Sec1/rbSec1、秀丽隐杆线虫 unc18 和酿酒酵母 Sec1p 分别具有 63%、53% 和 30% 的同一性。据报道，munc-18/n-Sec1/rbSec1 表达具有神经特异性，但 RNA 印迹分析表明，我们将其称为 muSec1（哺乳动物普遍存在的 Sec1）的新异构体普遍表达。我们还从 MIN6 cDNA 文库中鉴定出小鼠 munc-18/n-Sec1/rbSec1，表明参与囊泡运输的蛋白质的不同亚型存在于单个细胞中。 muSec1 与谷胱甘肽 S-转移酶-突触蛋白 1A 结合，并且与谷胱甘肽 S-转移酶-突触蛋白 4 融合蛋白结合，尽管亲和力较低。这些发现表明，muSec1 通过与突触融合蛋白家族结合，参与从高尔基体到质膜的蛋白质运输，并且从酵母到几乎所有哺乳动物细胞，蛋白质运输的基本机制都是保守的。
munc-18/n-Sec1/rbSec1, a brain homologue of the yeast Sec1p protein, is thought to participate in regulating the docking and fusion of synaptic vesicles. We have screened the mouse cDNA library of an MIN6 cell line, derived from pancreatic β cells, for its novel isoform and have identified a cDNA encoding a 593-amino acid protein having 63, 53, and 30% identity with munc-18/n-Sec1/rbSec1, Caenorhabditis elegans unc18, and Saccharomycescerevisiae Sec1p, respectively. While munc-18/n-Sec1/rbSec1 expression has been reported to be neural-specific, RNA blot analysis has revealed that the novel isoform, which we refer to as muSec1 (mammalian ubiquitous Sec1), is expressed ubiquitously. We have also identified mouse munc-18/n-Sec1/rbSec1 from the MIN6 cDNA library, indicating that different isoforms of a protein participating in vesicular transport exist in a single cell. muSec1 bound to glutathione S-transferase-syntaxin 1A and, although with lower affinity, to glutathione S-transferase-syntaxin 4 fusion protein. These findings suggest that muSec1 is, via its binding to the syntaxin family, involved in the protein trafficking from the Golgi apparatus to the plasma membrane and that the fundamental mechanisms of protein trafficking have been conserved from yeast through virtually all mammalian cells.
DOI：
10.1016/s0021-9258(19)49748-2
发表时间：
1992-06
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Cynthia A. Corley;Cain;William S Trimblez;Gustav E Lienhardq
通讯作者：
Cynthia A. Corley;Cain;William S Trimblez;Gustav E Lienhardq
完整大鼠脂肪细胞中胰岛素敏感己糖转运蛋白的光亲和标记。
DOI：
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发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Oka,Y;Czech,MP
通讯作者：
Czech,MP
DOI：
10.1073/pnas.91.6.2003
发表时间：
1994-03-15
影响因子：
11.1
作者：
GARCIA, EP;GATTI, E;DECAMILLI, P
通讯作者：
DECAMILLI, P